Xia Liu1, Yingying Li1, Yuting Wang1, Qinghong Wang2, Xin Li2, Yang Bi1, Lan Liu3, Xiaoping Wei1, Tingyu Li1, Jie Chen4. 1. Children Nutrition Research Center, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009 CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China. 2. Institute of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China. 3. Laboratory of Intestinal Physiology and Pathology, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA. 4. Children Nutrition Research Center, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009 CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China. Electronic address: jchen010@gmail.com.
Abstract
OBJECTIVES: Vitamin A (VA) is a critical micronutrient for life, especially during growth and development. There is a close relationship between VA deficiency (VAD) and the morbidity of diarrhea in the clinical setting. However, the regulatory mechanisms of VA are not clearly understood. METHODS: Specific-pathogen-free Wistar rats received a diet with or without VA before gestation. The offspring were submitted to an abdominal injection of Escherichia coli lipopolysaccharide. After the challenge, which lasted for 12 h, the serum retinol was detected by high-performance liquid chromatography, and the level of immunoglobulin A in the stool was analyzed by enzyme-linked immunosorbent assay. The lymphocyte immunophenotypes were evaluated with the use of flow cytometry with samples collected from the spleen, the mesenteric lymph nodes, Peyer patches, and intestinal intraepithelial lymphocytes. RESULTS: Early life VAD, independent of the lipopolysaccharide challenge, significantly decreased serum retinol level and CD8(+) intestinal intraepithelial lymphocytes. The level of immunoglobulin A secretion and percentages of splenic CD4(+)CD8(+) T cells were affected by the interaction effects of the lipopolysaccharide challenge and VAD treatment. Gestational VAD significantly increased the percentages of B cells in the mesenteric lymph nodes and decreased the percentages of CD11 C(+) dendritic cells and CD4(+)CD25(+) T cells from the Peyer patches. The lipopolysaccharide challenge only significantly increased percentages of splenic CD4(+)CD25(+) T cells. The intestinal tissue of the pups with VAD displayed mild inflammation. CONCLUSIONS: Gestational or early life VAD decreases the numbers of immune cells in offspring, which may partly suppress the activities of the mucosal immune responses in the intestine. This suggests that more attention should be given to the VA nutritional state of children and women of reproductive age.
OBJECTIVES:Vitamin A (VA) is a critical micronutrient for life, especially during growth and development. There is a close relationship between VA deficiency (VAD) and the morbidity of diarrhea in the clinical setting. However, the regulatory mechanisms of VA are not clearly understood. METHODS: Specific-pathogen-free Wistar rats received a diet with or without VA before gestation. The offspring were submitted to an abdominal injection of Escherichia colilipopolysaccharide. After the challenge, which lasted for 12 h, the serum retinol was detected by high-performance liquid chromatography, and the level of immunoglobulin A in the stool was analyzed by enzyme-linked immunosorbent assay. The lymphocyte immunophenotypes were evaluated with the use of flow cytometry with samples collected from the spleen, the mesenteric lymph nodes, Peyer patches, and intestinal intraepithelial lymphocytes. RESULTS: Early life VAD, independent of the lipopolysaccharide challenge, significantly decreased serum retinol level and CD8(+) intestinal intraepithelial lymphocytes. The level of immunoglobulin A secretion and percentages of splenic CD4(+)CD8(+) T cells were affected by the interaction effects of the lipopolysaccharide challenge and VAD treatment. Gestational VAD significantly increased the percentages of B cells in the mesenteric lymph nodes and decreased the percentages of CD11 C(+) dendritic cells and CD4(+)CD25(+) T cells from the Peyer patches. The lipopolysaccharide challenge only significantly increased percentages of splenic CD4(+)CD25(+) T cells. The intestinal tissue of the pups with VAD displayed mild inflammation. CONCLUSIONS: Gestational or early life VAD decreases the numbers of immune cells in offspring, which may partly suppress the activities of the mucosal immune responses in the intestine. This suggests that more attention should be given to the VA nutritional state of children and women of reproductive age.