Ya-si Xu1, Jian-guo Feng2, Dan Zhang3, Bo Zhang4, Min Luo3, Dan Su5, Neng-ming Lin4. 1. 1] College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China [2] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China. 2. Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China. 3. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China. 4. 1] Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China [2] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China. 5. 1] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China [2] College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China.
Abstract
AIM: To investigate the effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on human ovarian cancer cells in vitro. METHODS: Human epithelial ovarian cancer cell line A2780 was tested. Cell proliferation was examined with CCK-8 and colony formation assays. Cell cycle was analyzed with flow cytometry. Cell apoptosis was studied using Hoechst 33258 staining and Annexin V/PI staining with flow cytometry. The migration and invasion of A2780 cells were examined with transwell and wound healing assays. The expression of relevant proteins was detected with Western blot assays. RESULTS: SAC (1-100 mmol/L) inhibited the proliferation of A2780 cells in dose- and time-dependent manners (the IC50 value was approximately 25 mmol/L at 48 h, and less than 6.25 mmol/L at 96 h). Furthermore, SAC dose-dependently inhibited the colony formation of A2780 cells. Treatment of A2780 cells with SAC resulted in G1/S phase arrest and induced apoptosis, accompanied by decreased expression of pro-caspase-3, Parp-1 and Bcl-2, and increased expression of active caspase-3 and Bax. SAC treatment significantly reduced the migration of A2780 cells, and markedly decreased the protein expression of Wnt5a, p-AKT and c-Jun, which were the key proteins involved in proliferation and metastasis. CONCLUSION: SAC suppresses proliferation and induces apoptosis in A2780 ovarian cancer cells in vitro.
AIM: To investigate the effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on humanovarian cancer cells in vitro. METHODS:Humanepithelial ovarian cancer cell line A2780 was tested. Cell proliferation was examined with CCK-8 and colony formation assays. Cell cycle was analyzed with flow cytometry. Cell apoptosis was studied using Hoechst 33258 staining and Annexin V/PI staining with flow cytometry. The migration and invasion of A2780 cells were examined with transwell and wound healing assays. The expression of relevant proteins was detected with Western blot assays. RESULTS:SAC (1-100 mmol/L) inhibited the proliferation of A2780 cells in dose- and time-dependent manners (the IC50 value was approximately 25 mmol/L at 48 h, and less than 6.25 mmol/L at 96 h). Furthermore, SAC dose-dependently inhibited the colony formation of A2780 cells. Treatment of A2780 cells with SAC resulted in G1/S phase arrest and induced apoptosis, accompanied by decreased expression of pro-caspase-3, Parp-1 and Bcl-2, and increased expression of active caspase-3 and Bax. SAC treatment significantly reduced the migration of A2780 cells, and markedly decreased the protein expression of Wnt5a, p-AKT and c-Jun, which were the key proteins involved in proliferation and metastasis. CONCLUSION:SAC suppresses proliferation and induces apoptosis in A2780 ovarian cancer cells in vitro.
Authors: Yuta Kanamori; Lisa Dalla Via; Alberto Macone; Gianluca Canettieri; Antonio Greco; Antonio Toninello; Enzo Agostinelli Journal: Exp Ther Med Date: 2019-12-27 Impact factor: 2.447