| Literature DB >> 24360884 |
Wei Li1, Xin Li2, Tianda Li1, Ming-Gui Jiang3, Haifeng Wan1, Guan-Zheng Luo4, Chunjing Feng5, Xiaolong Cui6, Fei Teng5, Yan Yuan7, Quan Zhou7, Qi Gu2, Ling Shuai2, Jiahao Sha8, Yamei Xiao9, Liu Wang1, Zhonghua Liu10, Xiu-Jie Wang4, Xiao-Yang Zhao1, Qi Zhou11.
Abstract
The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat.Entities:
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Year: 2013 PMID： 24360884 DOI： 10.1016/j.stem.2013.11.016
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633