Literature DB >> 24357004

Chemoenzymatic bio-orthogonal chemistry for site-specific double modification of recombinant thrombomodulin.

Rui Jiang1, Lin Wang, Jacob Weingart, Xue-Long Sun.   

Abstract

Best of both worlds: A one-pot strategy for site-specific PEGylation through strain-promoted alkyne-azide cycloaddition (SPAAC) and fluorescent labeling through sortase A-mediated ligation (SML) of recombinant thrombomodulin without prior chemical modification and without diminishing the protein activity has been developed.
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  PEGylation; cycloadditions; fluorescent labeling; ligations; one-pot syntheses; thrombomodulin

Mesh：

Substances：

Year:  2013        PMID： 24357004      PMCID： PMC3926800          DOI： 10.1002/cbic.201300641

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  19 in total

1.  Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry.

Authors:  William P Heal; Megan H Wright; Emmanuelle Thinon; Edward W Tate
Journal:  Nat Protoc       Date:  2011-12-22       Impact factor: 13.491

2.  Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation.

Authors:  Stephan Pritz; Yvonne Wolf; Oliver Kraetke; Jana Klose; Michael Bienert; Michael Beyermann
Journal:  J Org Chem       Date:  2007-04-14       Impact factor: 4.354

3.  Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site.

Authors:  Yuichi Yamamura; Hidehiko Hirakawa; Satoshi Yamaguchi; Teruyuki Nagamune
Journal:  Chem Commun (Camb)       Date:  2011-03-15       Impact factor: 6.222

4.  In vitro anticoagulant properties of a minimum functional fragment of human thrombomodulin and in vivo demonstration of its benefit as an anticoagulant in extracorporeal circulation using a monkey model.

Authors:  M Suzuki; M Mohri; S Yamamoto
Journal:  Thromb Haemost       Date:  1998-02       Impact factor: 5.249

5.  The last three consecutive epidermal growth factor-like structures of human thrombomodulin comprise the minimum functional domain for protein C-activating cofactor activity and anticoagulant activity.

Authors:  M Zushi; K Gomi; S Yamamoto; I Maruyama; T Hayashi; K Suzuki
Journal:  J Biol Chem       Date:  1989-06-25       Impact factor: 5.157

6.  End-point immobilization of recombinant thrombomodulin via sortase-mediated ligation.

Authors:  Rui Jiang; Jacob Weingart; Hailong Zhang; Yong Ma; Xue-Long Sun
Journal:  Bioconjug Chem       Date:  2012-03-08       Impact factor: 4.774

Review 7.  Thrombomodulin links coagulation to inflammation and immunity.

Authors:  John Morser
Journal:  Curr Drug Targets       Date:  2012-03       Impact factor: 3.465

8.  Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif.

Authors:  H Ton-That; G Liu; S K Mazmanian; K F Faull; O Schneewind
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-26       Impact factor: 11.205

9.  Sortase-Mediated Transpeptidation for Site-Specific Modification of Peptides, Glycopeptides, and Proteins.

Authors:  Zhimeng Wu; Zhongwu Guo
Journal:  J Carbohydr Chem       Date:  2012-01-19       Impact factor: 1.667

10.  Bioorthogonal chemistry: fishing for selectivity in a sea of functionality.

Authors:  Ellen M Sletten; Carolyn R Bertozzi
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336
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  4 in total

1.  End-point modification of recombinant thrombomodulin with enhanced stability and anticoagulant activity.

Authors:  Xia Liu; Mallorie Boron; Yu Zhao; Xue-Long Sun
Journal:  Eur J Pharm Sci       Date:  2019-09-09       Impact factor: 4.384

2.  Layer-by-Layer Heparinization of the Cell Surface by Using Heparin-Binding Peptide Functionalized Human Serum Albumin.

Authors:  Guowei Song; Yaning Hu; Yusheng Liu; Rui Jiang
Journal:  Materials (Basel)       Date:  2018-05-20       Impact factor: 3.623

Review 3.  Broadening the scope of sortagging.

Authors:  Xiaolin Dai; Alexander Böker; Ulrich Glebe
Journal:  RSC Adv       Date:  2019-02-06       Impact factor: 4.036

4.  A versatile papaya mosaic virus (PapMV) vaccine platform based on sortase-mediated antigen coupling.

Authors:  Ariane Thérien; Mikaël Bédard; Damien Carignan; Gervais Rioux; Louis Gauthier-Landry; Marie-Ève Laliberté-Gagné; Marilène Bolduc; Pierre Savard; Denis Leclerc
Journal:  J Nanobiotechnology       Date:  2017-07-18       Impact factor: 10.435
  4 in total

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