Binding site of neutralizing monoclonal antibodies obtained after in vivo priming with purified VP1 of poliovirus type 1 is located between amino acid residues 93 and 104 of VP1.

Three hybridomas obtained after in vitro stimulation of spleen cells of mice primed in vivo with purified VP1 of poliovirus type 1 (Mahoney) with the homologous virus produced antibodies which reacted with VP1 and immunoprecipitated and neutralized only the homologous virus. Evidence for the location of their binding sites was obtained by inhibition of virus neutralization and virus binding by a synthetic peptide comprising the amino acid sequence 93-104 of VP1 of poliovirus type 1 (Mahoney).