Literature DB >> 24343756

A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry.

Ayan Samanta1, André Krause, Andres Jäschke.   

Abstract

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5'-end, and thus through ligation the terminal label can be transformed to an internal one.

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Year:  2014        PMID： 24343756     DOI： 10.1039/c3cc46132g

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  10 in total

1.  Polymerase-Mediated Site-Specific Incorporation of a Synthetic Fluorescent Isomorphic G Surrogate into RNA.

Authors:  Yao Li; Andrea Fin; Lisa McCoy; Yitzhak Tor
Journal:  Angew Chem Int Ed Engl       Date:  2016-12-21       Impact factor: 15.336

2.  Metabolic Incorporation of Azide Functionality into Cellular RNA.

Authors:  Sarah Nainar; Samantha Beasley; Michael Fazio; Miles Kubota; Nan Dai; Ivan R Corrêa; Robert C Spitale
Journal:  Chembiochem       Date:  2016-09-30       Impact factor: 3.164

3.  Nucleotidyl transferase assisted DNA labeling with different click chemistries.

Authors:  Marie-Luise Winz; Eva Christina Linder; Timon André; Juliane Becker; Andres Jäschke
Journal:  Nucleic Acids Res       Date:  2015-05-26       Impact factor: 16.971

4.  A novel route for preparing 5' cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry.

Authors:  Sylwia Walczak; Anna Nowicka; Dorota Kubacka; Kaja Fac; Przemyslaw Wanat; Seweryn Mroczek; Joanna Kowalska; Jacek Jemielity
Journal:  Chem Sci       Date:  2016-08-16       Impact factor: 9.825

5.  Synthesis of a bifunctional cytidine derivative and its conjugation to RNA for in vitro selection of a cytidine deaminase ribozyme.

Authors:  Nico Rublack; Sabine Müller
Journal:  Beilstein J Org Chem       Date:  2014-08-15       Impact factor: 2.883

6.  A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA.

Authors:  Anupam A Sawant; Arun A Tanpure; Progya P Mukherjee; Soumitra Athavale; Ashwin Kelkar; Sanjeev Galande; Seergazhi G Srivatsan
Journal:  Nucleic Acids Res       Date:  2015-09-17       Impact factor: 16.971

Review 7.  Synthetic mRNA capping.

Authors:  Fabian Muttach; Nils Muthmann; Andrea Rentmeister
Journal:  Beilstein J Org Chem       Date:  2017-12-20       Impact factor: 2.883

8.  A Novel NAD-RNA Decapping Pathway Discovered by Synthetic Light-Up NAD-RNAs.

Authors:  Florian Abele; Katharina Höfer; Patrick Bernhard; Julia Grawenhoff; Maximilian Seidel; André Krause; Sara Kopf; Martin Schröter; Andres Jäschke
Journal:  Biomolecules       Date:  2020-03-28

9.  Post-transcriptional labeling by using Suzuki-Miyaura cross-coupling generates functional RNA probes.

Authors:  Manisha B Walunj; Arun A Tanpure; Seergazhi G Srivatsan
Journal:  Nucleic Acids Res       Date:  2018-06-20       Impact factor: 16.971

Review 10.  Strategies for Covalent Labeling of Long RNAs.

Authors:  Hannah Depmeier; Eva Hoffmann; Lisa Bornewasser; Stephanie Kath-Schorr
Journal:  Chembiochem       Date:  2021-06-17       Impact factor: 3.164
  10 in total

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