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Literature DB >> 24336506

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe.

Hong-Zhang He¹, Ka-Ho Leung, Wei Wang, Daniel Shiu-Hin Chan, Chung-Hang Leung, Dik-Lung Ma.

Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

Entities: Chemical Gene

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Year: 2013 PMID： 24336506 DOI： 10.1039/c3cc47444e

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

10 in total

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Authors: Chun-Yuen Wong; Lai-Hon Chung; Sheng Lin; Daniel Shiu-Hin Chan; Chung-Hang Leung; Dik-Lung Ma
Journal: Sci Rep Date: 2014-11-20 Impact factor: 4.379

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe.

Review 1. Fluorescent Probes of DNA Repair.

2. A colorimetric and luminescent dual-modal assay for Cu(II) ion detection using an iridium(III) complex.

3. A ruthenium(II) complex supported by trithiacyclononane and aromatic diimine ligand as luminescent switch-on probe for biomolecule detection and protein staining.

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5. A colorimetric chemosensor for Cu²⁺ ion detection based on an iridium(III) complex.

6. A cyclometalated iridium(III) complex used as a conductor for the electrochemical sensing of IFN-γ.

7. Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor.

8. Isothermal titration calorimetric assessment of lignin conversion by laccases.

9. Accurate label-free reaction kinetics determination using initial rate heat measurements.

10. Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine.