| Literature DB >> 24334120 |
Maiyu Xu1, Yi Wang2, Lei Chen1, Bujian Pan1, Feng Chen1, Yang Fang3, Zhengping Yu4, Gang Chen5.
Abstract
Ribosomal protein s15a (RPS15A) is a highly conserved protein that promotes mRNA/ribosome interactions early in translation. Recent evidence showed that RPS15A could stimulate growth in yeast, plant and human lung carcinoma. Here we report that RPS15A knockdown could inhibit hepatic cancer cell growth in vitro. When transduced with shRPS15A-containing lentivirus, we observed inhibited cell proliferation and impaired colony formation in both HepG2 and Bel7404 cells. Furthermore, cell cycle analysis showed that HepG2 cells were arrested at the G0/G1 phase when transduced with Lv-shRPS15A. In conclusion, our findings provide for the first time the biological effects of RPS15A in hepatic cancer cell growth. RPS15A may play a prominent role in heptocarcinogenesis and serve as a potential therapeutic target in hepatocellular carcinoma.Entities:
Keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ATCC; American Type Culture Collection; DMEM; Dulbecco's modified Eagle's medium; FACS; FBS; GFP; Growth; HBxAg; HCC; Hepatocellular carcinoma; MTT; PBS; PI; RNA interference; RNAi; RPS15A; TGFβ1; eIF-4F; eukaryotic initiation factor 4F; fetal bovine serum; fluorescence-activated cell sorting; green fluorescence protein; hepatitis B virus×antigen; hepatocellular carcinoma; phosphate-buffered saline; propidium iodide; ribosomal protein S15A; shRNA; shRPS15A; short hairpin RNA; siRNA; small interfering RNA; transforming growth factor beta 1
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Year: 2013 PMID： 24334120 DOI： 10.1016/j.gene.2013.11.075
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688