Zhengyu Fang1, Lamei Wu2, Luo Wang1, Yang Yang1, Yusheng Meng2, Hongyu Yang3. 1. Biomedical Research Institute, Shenzhen-Peking University-Hongkong University Science and Technology (PKU-HKUST) Medical Center, Shenzhen, Guangdong Province, China. 2. Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, China. 3. Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, China. Electronic address: yanghongyu_1979@163.com.
Abstract
OBJECTIVE: The aim of this study was to examine the expression of several cancer-related long non-coding RNAs (lncRNAs) in patients with tongue squamous cell carcinoma (TSCC) and to explore its clinical significance. STUDY DESIGN: A total of 94 TSCC tissue specimens and matched adjacent normal tissue specimens were collected from patients undergoing surgery for TSCC. Differences in the expression of cancer-related lncRNAs were examined via quantitative reverse transcriptase polymerase chain reaction. WST-1 assay and transwell migration assay were carried out to estimate the proliferation and migration abilities of TSCC cells with different expression levels of urothelial cancer-associated 1 (UCA1) lncRNA. RESULTS: The expression levels of lncRNA UCA1 were significantly elevated in TSCC tissues (P < .0001) and were statistically correlated with lymph node metastasis (P = .0371). Over-expression of UCA1 lncRNA could promote metastatic but not proliferation ability of TSCC cells. CONCLUSIONS: Expression of UCA1 lncRNA was enhanced in TSCCs and may play a role in tumor metastasis.
OBJECTIVE: The aim of this study was to examine the expression of several cancer-related long non-coding RNAs (lncRNAs) in patients with tongue squamous cell carcinoma (TSCC) and to explore its clinical significance. STUDY DESIGN: A total of 94 TSCC tissue specimens and matched adjacent normal tissue specimens were collected from patients undergoing surgery for TSCC. Differences in the expression of cancer-related lncRNAs were examined via quantitative reverse transcriptase polymerase chain reaction. WST-1 assay and transwell migration assay were carried out to estimate the proliferation and migration abilities of TSCC cells with different expression levels of urothelial cancer-associated 1 (UCA1) lncRNA. RESULTS: The expression levels of lncRNA UCA1 were significantly elevated in TSCC tissues (P < .0001) and were statistically correlated with lymph node metastasis (P = .0371). Over-expression of UCA1 lncRNA could promote metastatic but not proliferation ability of TSCC cells. CONCLUSIONS: Expression of UCA1 lncRNA was enhanced in TSCCs and may play a role in tumor metastasis.