Patricia Becerra1, Domenico Ricucci2, Simona Loghin3, Jennifer L Gibbs4, Louis M Lin4. 1. Department of Endodontics, Faculty of Dentistry, University Santo Tomás in Bogotá, Bogotá, Colombia. 2. Private Practice, Cetraro, Italy. Electronic address: dricucci@libero.it. 3. Private Practice, Cetraro, Italy. 4. Department of Endodontics, New York University, New York, New York.
Abstract
INTRODUCTION: Histologic studies of teeth from animal models of revascularization/revitalization are available; however, specimens from human studies are lacking. The nature of tissues formed in the canal of human revascularized/revitalized teeth was not well established. METHODS: An immature mandibular premolar with infected necrotic pulp and a chronic apical abscess was treated with revascularization/revitalization procedures. At both the 18-month and 2-year follow-up visits, radiographic examination showed complete resolution of the periapical lesion, narrowing of the root apex without root lengthening, and minimal thickening of the canal walls. The revascularized/revitalized tooth was removed because of orthodontic treatment and processed for histologic examination. RESULTS: The large canal space of revascularized/revitalized tooth was not empty and filled with fibrous connective tissue. The apical closure was caused by cementum deposition without dentin. Some cementum-like tissue was formed on the canal dentin walls. Inflammatory cells were observed in the coronal and middle third of revascularized/revitalized tissue. CONCLUSIONS: In the present case, the tissue formed in the canal of a human revascularized/revitalized tooth was soft connective tissue similar to that in the periodontal ligament and cementum-like or bone-like hard tissue, which is comparable with the histology observed in the canals of teeth from animal models of revascularization/revitalization.
INTRODUCTION: Histologic studies of teeth from animal models of revascularization/revitalization are available; however, specimens from human studies are lacking. The nature of tissues formed in the canal of human revascularized/revitalized teeth was not well established. METHODS: An immature mandibular premolar with infected necrotic pulp and a chronic apical abscess was treated with revascularization/revitalization procedures. At both the 18-month and 2-year follow-up visits, radiographic examination showed complete resolution of the periapical lesion, narrowing of the root apex without root lengthening, and minimal thickening of the canal walls. The revascularized/revitalized tooth was removed because of orthodontic treatment and processed for histologic examination. RESULTS: The large canal space of revascularized/revitalized tooth was not empty and filled with fibrous connective tissue. The apical closure was caused by cementum deposition without dentin. Some cementum-like tissue was formed on the canal dentin walls. Inflammatory cells were observed in the coronal and middle third of revascularized/revitalized tissue. CONCLUSIONS: In the present case, the tissue formed in the canal of a human revascularized/revitalized tooth was soft connective tissue similar to that in the periodontal ligament and cementum-like or bone-like hard tissue, which is comparable with the histology observed in the canals of teeth from animal models of revascularization/revitalization.
Authors: Adel S Alobaid; Lina M Cortes; Jeffery Lo; Thuan T Nguyen; Jeffery Albert; Abdulaziz S Abu-Melha; Louis M Lin; Jennifer L Gibbs Journal: J Endod Date: 2014-06-13 Impact factor: 4.171
Authors: Alaa H A Sabrah; Ghaeth H Yassen; Wai-Ching Liu; W Scott Goebel; Richard L Gregory; Jeffrey A Platt Journal: Clin Oral Investig Date: 2015-02-19 Impact factor: 3.573
Authors: Tarek Mohamed A Saoud; Ashraf Zaazou; Ahmed Nabil; Sybel Moussa; Louis M Lin; Jennifer L Gibbs Journal: J Endod Date: 2014-10-16 Impact factor: 4.171
Authors: Nastaran Meschi; Petra Hilkens; Ivo Lambrichts; Kathleen Van den Eynde; Athina Mavridou; Olaf Strijbos; Marieke De Ketelaere; Gertrude Van Gorp; Paul Lambrechts Journal: Clin Oral Investig Date: 2015-08-08 Impact factor: 3.573