Maria Elena Gallina1, Jianmin Xu, Thomas Dertinger, Adva Aizer, Yaron Shav-Tal, Shimon Weiss. 1. Department of Chemistry and Biochemistry, Department of Physiology, and the California NanoSystem Institute (CNSI), University of California Los Angeles, Los Angeles, CA 90095-1569, USA ; Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, Bologna 40126, Italy.
Abstract
BACKGROUND: Multi-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructura relationships between- and provide co-localization information of- different proteins inside the cell or even within organelles at a higher resolution than afforded by conventional diffraction-limited imaging. While still very challenging, important SR colocalization results have been reported in recent years using STED, PALM and STORM techniques. RESULTS: In this work, we demonstrate dual-color Super Resolution Optical Fluctuations Imaging (SOFI) using a standard far-field fluorescence microscope and different color blinking quantum dots. We define the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network. Our finding could open up new perspectives on the role of the cytoskeleton in PB formation and assembly. Further insights into PB internal organization are also reported and discussed. CONCLUSIONS: Our results demonstrate the suitability and facile use of multi-color SOFI for the investigation of intracellular ultrastructures.
BACKGROUND: Multi-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructura relationships between- and provide co-localization information of- different proteins inside the cell or even within organelles at a higher resolution than afforded by conventional diffraction-limited imaging. While still very challenging, important SR colocalization results have been reported in recent years using STED, PALM and STORM techniques. RESULTS: In this work, we demonstrate dual-color Super Resolution Optical Fluctuations Imaging (SOFI) using a standard far-field fluorescence microscope and different color blinking quantum dots. We define the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network. Our finding could open up new perspectives on the role of the cytoskeleton in PB formation and assembly. Further insights into PB internal organization are also reported and discussed. CONCLUSIONS: Our results demonstrate the suitability and facile use of multi-color SOFI for the investigation of intracellular ultrastructures.
Authors: Thomas Dertinger; Ryan Colyer; Robert Vogel; Mike Heilemann; Markus Sauer; Jörg Enderlein; Shimon Weiss Journal: Adv Exp Med Biol Date: 2012 Impact factor: 2.622
Authors: Jennifer M Urban; Wesley Chiang; Jennetta W Hammond; Nicole M B Cogan; Angela Litzburg; Rebeckah Burke; Harry A Stern; Harris A Gelbard; Bradley L Nilsson; Todd D Krauss Journal: J Phys Chem B Date: 2021-03-08 Impact factor: 2.991