Literature DB >> 24316843

Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus.

Maren Thomsen1, Lilly Skalden, Gottfried J Palm, Matthias Höhne, Uwe T Bornscheuer, Winfried Hinrichs.   

Abstract

The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD phasing.

Entities:  

Keywords:  (R)-selective amine transaminase; S-SAD

Mesh：

Substances：

Year:  2013        PMID： 24316843      PMCID： PMC3855733          DOI： 10.1107/S1744309113030923

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


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