PURPOSE: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. METHODS: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. RESULTS: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. CONCLUSION: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR.
PURPOSE: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in humanbreast cancer MCF-7 cells. METHODS:Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. RESULTS: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. CONCLUSION: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR.
Entities:
Keywords:
Breast cancer; Folate receptor; Internalization; MCF-7 cells; Magnetic nanoparticles
Authors: Maria Moros; Beatriz Pelaz; Pilar López-Larrubia; Maria L García-Martin; Valeria Grazú; Jesus M de la Fuente Journal: Nanoscale Date: 2010-07-30 Impact factor: 7.790
Authors: Lihong Bu; Jin Xie; Kai Chen; Jing Huang; Zoraida P Aguilar; Andrew Wang; Kin Wai Sun; Mei-Sze Chua; Samuel So; Zhen Cheng; Henry S Eden; Baozhong Shen; Xiaoyuan Chen Journal: Contrast Media Mol Imaging Date: 2012 Jul-Aug Impact factor: 3.161
Authors: Manoj Kumar; Gurpal Singh; Vikas Arora; Sujeet Mewar; Uma Sharma; N R Jagannathan; Sameer Sapra; Amit K Dinda; Surender Kharbanda; Harpal Singh Journal: Int J Nanomedicine Date: 2012-07-06