| Literature DB >> 24311688 |
Xinhong Lim1, Si Hui Tan, Winston Lian Chye Koh, Rosanna Man Wah Chau, Kelley S Yan, Calvin J Kuo, Renée van Amerongen, Allon Moshe Klein, Roel Nusse.
Abstract
The skin is a classical example of a tissue maintained by stem cells. However, the identity of the stem cells that maintain the interfollicular epidermis and the source of the signals that control their activity remain unclear. Using mouse lineage tracing and quantitative clonal analyses, we showed that the Wnt target gene Axin2 marks interfollicular epidermal stem cells. These Axin2-expressing cells constitute the majority of the basal epidermal layer, compete neutrally, and require Wnt/β-catenin signaling to proliferate. The same cells contribute robustly to wound healing, with no requirement for a quiescent stem cell subpopulation. By means of double-labeling RNA in situ hybridization in mice, we showed that the Axin2-expressing cells themselves produce Wnt signals as well as long-range secreted Wnt inhibitors, suggesting an autocrine mechanism of stem cell self-renewal.Entities:
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Year: 2013 PMID： 24311688 PMCID： PMC4081860 DOI： 10.1126/science.1239730
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 63.714