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Literature DB >> 24309

Studies on glutamic-oxalacetic (GOT) and glutamic-pyruvic (GPT) transaminases of swine kidney worm Stephanurus dentatus (Diesing, 1839). I. Assay and general properties.

Abstract

Biochemical studies on the two transaminases GOT and GPT of swine kidney worm Stephanurus dentatus have been made. GOT has been found much more active than GPT. Enzyme activities are based on the formation of oxaloacetate (GOT) or pyruvate (GPT) from aspartic acid and alanine respectively with oxoglutarate. A linear relationship is observed between the enzyme concentration and activity. GOT shows a maximum activity at pH 8.0 and Michaelis constant 9 X 10(-3) M for male and 2.9 X 10(-3) M for female. GPT has an optimum pH of 7.5 and a Michaelis constant 19 X 10(-3) M for male and 8 X 10(-3) M for female. The optimum temperature for both GOT and GPT was 60 degrees C.

Entities: Chemical Disease Gene Species

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Year: 1977 PMID： 24309 DOI： 10.1007/BF00390116

Source DB: PubMed Journal: Z Parasitenkd ISSN： 0044-3255

14 in total

1. The metabolism of Ascaris lumbricoides ovaries. II. Amino acid metabolism.

Authors: J K POLLAK; D FAIRBAIRN
Journal: Can J Biochem Physiol Date: 1955-05

2. [Study on the constitution and protein metabolism of Ascaris lumbricoides].

Authors: J SAVEL
Journal: Rev Pathol Gen Physiol Clin Date: 1955-02

3. [Study of some aspects of the intermediate metabolism of amino acids in the swine ascarid (Ascaris lumbricoides L., 1758)].

Authors: R CAVIER; J SAVEL
Journal: Bull Soc Chim Biol (Paris) Date: 1954

8. Intermediary protein metabolism in helminths. II. Effect of host castration on amino acid metabolism in Hymenolepis diminuta.

Authors: D V ALDRICH; A C CHANDLER; J W DAUGHERTY
Journal: Exp Parasitol Date: 1954-03 Impact factor: 2.011

Studies on glutamic-oxalacetic (GOT) and glutamic-pyruvic (GPT) transaminases of swine kidney worm Stephanurus dentatus (Diesing, 1839). I. Assay and general properties.

1. The metabolism of Ascaris lumbricoides ovaries. II. Amino acid metabolism.

2. [Study on the constitution and protein metabolism of Ascaris lumbricoides].

3. [Study of some aspects of the intermediate metabolism of amino acids in the swine ascarid (Ascaris lumbricoides L., 1758)].

4. The gamma-aminobutyric acid-alpha-ketoglutaric acid transaminase of beef brain.

5. Glutamic-oxaloacetic transaminase of cauliflower. 2. Kinetics and mechanism of action.

6. Glutamic aspartic transaminase. I. Assay, purification, and general properties.

7. Studies on transaminases of Schistosoma japonicum.

8. Intermediary protein metabolism in helminths. II. Effect of host castration on amino acid metabolism in Hymenolepis diminuta.

9. Distribution of transaminases in rat organs.

10. Glutamate transaminase activities of the liver fluke, Fasciola hepatica (Linnaeus, 1758).

1. Effect of seasonal variations and cold acclimation on serum transaminase activity of common Indian frog Rana tigrina.