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Literature DB >> 2429900

Direct identification of small sequence changes in chromosomal DNA.

Abstract

Dideoxynucleotide chain termination sequencing has been applied directly to genomic DNA templates by annealing radiolabeled oligodeoxynucleotide primers to unique sites in total yeast DNA and extending with avian myoblastosis virus (AMV) reverse transcriptase. The technique is used here to confirm the introduction of selectively altered tRNA genes into the Saccharomyces cerevisiae genome by gene replacement.

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Year: 1986 PMID： 2429900 DOI： 10.1016/0378-1119(86)90056-9

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

11 in total

1. Characterization of RPR1, an essential gene encoding the RNA component of Saccharomyces cerevisiae nuclear RNase P.

Authors: J Y Lee; C E Rohlman; L A Molony; D R Engelke
Journal: Mol Cell Biol Date: 1991-02 Impact factor: 4.272

2. Localization of sequences required in cis for yeast Ty1 element transposition near the long terminal repeats: analysis of mini-Ty1 elements.

Authors: H Xu; J D Boeke
Journal: Mol Cell Biol Date: 1990-06 Impact factor: 4.272

3. Identification of mar mutations in herpes simplex virus type 1 glycoprotein B which alter antigenic structure and function in virus penetration.

Authors: S L Highlander; D J Dorney; P J Gage; T C Holland; W Cai; S Person; M Levine; J C Glorioso
Journal: J Virol Date: 1989-02 Impact factor: 5.103

Direct identification of small sequence changes in chromosomal DNA.

1. Characterization of RPR1, an essential gene encoding the RNA component of Saccharomyces cerevisiae nuclear RNase P.

2. Localization of sequences required in cis for yeast Ty1 element transposition near the long terminal repeats: analysis of mini-Ty1 elements.

3. Identification of mar mutations in herpes simplex virus type 1 glycoprotein B which alter antigenic structure and function in virus penetration.

4. Direct sequencing of enzymatically amplified human genomic DNA.

5. Comparison of tRNA gene transcription complexes formed in vitro and in nuclei.

6. Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5'-flanking region.

7. Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P.

8. Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species.

9. Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P from Saccharomyces cerevisiae.

10. Expression of RNase P RNA in Saccharomyces cerevisiae is controlled by an unusual RNA polymerase III promoter.