Reza Fekrazad1, Farzaneh Khoei, Neda Hakimiha, Abbas Bahador. 1. Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. METHODS: Standard Suspensions of S. mutans were treated in two groups of photodynamic therapy with Toluidine blue O and Rhadachlorin(®) and photothermal therapy by EmunDo(®) and their individual light sources, then Bacterial suspension from each treatment was subcultured on the surface of Mueller-Hinton agar plates and bacterial growth was assessed. The results were analyzed by analysis of variance and Tukey test (p<0.05). RESULTS: After treatments significant reduction of S. mutans viability in planktonic culture was observed in both groups of photodynamic and photothermal therapy with no priority. CONCLUSION: Photoelimination can be a novel modality in the eradication of S. mutans colonies in near future.
BACKGROUND: Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynamic and photothermal therapy. METHODS: Standard Suspensions of S. mutans were treated in two groups of photodynamic therapy with Toluidine blue O and Rhadachlorin(®) and photothermal therapy by EmunDo(®) and their individual light sources, then Bacterial suspension from each treatment was subcultured on the surface of Mueller-Hinton agar plates and bacterial growth was assessed. The results were analyzed by analysis of variance and Tukey test (p<0.05). RESULTS: After treatments significant reduction of S. mutans viability in planktonic culture was observed in both groups of photodynamic and photothermal therapy with no priority. CONCLUSION: Photoelimination can be a novel modality in the eradication of S. mutans colonies in near future.