Literature DB >> 242796

The characterization of phosphoseryl tRNA from lactating bovine mammary gland.

S J Sharp, T S Stewart.   

Abstract

BD-cellulose and RPC-5 chromatography of tRNA isolated from lactating bovine mammary gland showed the presence of four seryl-tRNA isoacceptors. The species, tRNA IV Ser, with the strongest affinity for BD-cellulose (required ethanol in the elution buffer) could be phosphorylated in the presence of serine, [gamma-32 P]-ATP, seryl-tRNA synthetase and phosphotransferase activity from the same tissue. O-Phosphoserine was identified as the 32P-labelled product after mild alkaline hydrolysis of this aminoacylated tRNA. Pancreatic ribonuclease treatment of the aminoacylated tRNA yielded a labelled product which was identified as phosphoseryladenosine. These results indicated there is a specific phosphoseryl tRNA species in lactating bovine mammary gland. It appears that the formation of phosphoseryl-tRNA proceeds by enzymic phosphorylation of seryl-tRNA.

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Year:  1977        PMID： 242796      PMCID： PMC342552          DOI： 10.1093/nar/4.7.2123

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

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3.  Casein kinase from the Golgi apparatus of lactating mammary gland.

Authors:  E W Bingham; H M Farrel
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Authors:  W F Bridgers
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5.  Isoaccepting +RNA's in mouse plasma cell tumors that synthesize different myeloma protein.

Authors:  W K Yang; G D Novelli
Journal:  Biochem Biophys Res Commun       Date:  1968-05-23       Impact factor: 3.575

6.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857

7.  Phosphorylation of casein. Role of the golgi apparatus.

Authors:  E W Bingham; H M Farrell; J J Basch
Journal:  J Biol Chem       Date:  1972-12-25       Impact factor: 5.157

8.  Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids.

Authors:  A D Kelmers; D E Heatherly
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9.  Casein biosynthesis: evidence for phosphorylation of precursor proteins.

Authors:  R W Turkington; Y J Topper
Journal:  Biochim Biophys Acta       Date:  1966-10-31

10.  Participation of aminoacyl transfer ribonucleic acid in aminoacyl phosphatidylglycerol synthesis. II. Specificity of alanyl phosphatidylglycerol synthetase.

Authors:  R M Gould; M P Thornton; V Liepkalns; W J Lennarz
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157
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  15 in total

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3.  Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing.

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Authors:  D Hatfield; A Diamond; B Dudock
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Review 8.  Amino acid modifications on tRNA.

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9.  Phosphate incorporation into secretory protein of Chironomus salivary glands occurs during translation.

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10.  The leaky UGA termination codon of tobacco rattle virus RNA is suppressed by tobacco chloroplast and cytoplasmic tRNAs(Trp) with CmCA anticodon.

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