Peroxidase and leucine-aminopeptidase in diploidMedicago species closely related to alfalfa: Multiple gene loci, multiple allelism, and linkage.

The genetics of peroxidase and leucine-aminopeptidase isozymes was studied utilizing starch gel electrophoresis in the diploidsMedicago sativa L. (M. coerulea Less.) andM. falcata L. Three anodal and one cathodal sets of peroxidase isozymes identify four linked loci. In addition, two anodal sets of leucine-aminopeptidase isozymes identify two loci that may be linked. The allozymes at each of the loci segregated as expected for monomeric enzymes. However in several crosses there were deficiencies in the number of progeny in particular genotypic classes. This could result from the segregation of recessive deleterious genes linked to some of the allozyme alleles. This is the first report of multiple loci and multiple alleles determining isozymes inMedicago.