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Literature DB >> 24213496

Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants.

Abstract

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.

Entities: Chemical Species

Year: 1992 PMID： 24213496 DOI： 10.1007/BF00235264

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

13 in total

1. Expression of genes transferred into monocot and dicot plant cells by electroporation.

Authors: M Fromm; L P Taylor; V Walbot
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

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Authors: C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal: Science Date: 1988-04-08 Impact factor: 47.728

3. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

4. Factors affecting transient gene expression in electroporated black spruce (Picea mariana) and jack pine (Pinus banksiana) protoplasts.

Authors: T E Tautorus; F Bekkaoui; M Pilon; R S Datla; W L Crosby; L C Fowke; D I Dunstan
Journal: Theor Appl Genet Date: 1989-10 Impact factor: 5.699

5. Genotype- and promoter-induced variability in transient β-glucuronidase expression in pea protoplasts.

Authors: S L Hobbs; J A Jackson; D S Baliski; C M Delong; J D Mahon
Journal: Plant Cell Rep Date: 1990-06 Impact factor: 4.570

6. Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system.

Authors: N S Nehra; R N Chibbar; K K Kartha; R S Datla; W L Crosby; C Stushnoff
Journal: Plant Cell Rep Date: 1990-10 Impact factor: 4.570

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Authors: F Bekkaoui; M Pilon; E Laine; D S Raju; W L Crosby; D I Dunstan
Journal: Plant Cell Rep Date: 1988-12 Impact factor: 4.570

8. Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species.

Authors: R M Hauptmann; P Ozias-Akins; V Vasil; Z Tabaeizadeh; S G Rogers; R B Horsch; I K Vasil; R T Fraley
Journal: Plant Cell Rep Date: 1987-07 Impact factor: 4.570

9. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

Authors: L Menczel; F Nagy; Z R Kiss; P Maliga
Journal: Theor Appl Genet Date: 1981-03 Impact factor: 5.699

3. A Protoplast Transient Expression System to Enable Molecular, Cellular, and Functional Studies in Phalaenopsis orchids.

Authors: Hsiang-Yin Lin; Jhun-Chen Chen; Su-Chiung Fang
Journal: Front Plant Sci Date: 2018-06-22 Impact factor: 5.753

3 in total