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Literature DB >> 24272394

Plant phosphoglucose isomerase genes lack introns and are expressed in Escherichia coli.

R C Tait¹, B E Froman, D L Laudencia-Chingcuanco, L D Gottlieb.

Abstract

Nuclear genes that appear to encode both cytosolic and plastid isozymes of phosphoglucose isomerase (PGI), an essential glycolytic enzyme, have been isolated from three diploid species of the annual wild flower genus Clarkia (Onagraceae). The genes do not contain introns and are expressed to varying degrees in Escherichia coli when cloned in either Charon 35 phage or pUC plasmid vectors. The PGI proteins synthesized in E. coli form dimers, are catalytically active, and their electrophoretic mobilities are similar to those of appropriate Clarkia PGIs. The nucleotide sequence of a gene encoding a plastid isozyme of C. unguiculata is described.

Entities: Disease Species

Year: 1988 PMID： 24272394 DOI： 10.1007/BF00039018

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

25 in total

8. Evidence from subunit molecular weight suggests hybridization was the source of the phosphoglucose isomerase gene duplication in Clarkia.

Authors: L D Gottlieb; R C Higgins
Journal: Theor Appl Genet Date: 1984-07 Impact factor: 5.699

9. Isoenzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinis communis L.

Authors: P D Simcox; D T Dennis
Journal: Plant Physiol Date: 1978-06 Impact factor: 8.340

Plant phosphoglucose isomerase genes lack introns and are expressed in Escherichia coli.

1. GENE DUPLICATION AND PHYLOGENY IN CLARKIA.

2. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.

3. Two isoenzymes of glucosephosphate isomerase from spinach leaves and their intracellular compartmentation.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Phosphoglucose Isomerase Expression in Species of Clarkia with and without a Duplication of the Coding Gene.

6. Efficient isolation of genes by using antibody probes.

7. Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts.

8. Evidence from subunit molecular weight suggests hybridization was the source of the phosphoglucose isomerase gene duplication in Clarkia.

9. Isoenzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinis communis L.

10. Subunit hybridization and immunological studies of duplicated phosphoglucose isomerase isozymes.

1. Anomalous phylogeny involving the enzyme glucose-6-phosphate isomerase.

2. Molecular analysis of the plant gene encoding cytosolic phosphoglucose isomerase.

3. Transkingdom transfer of the phosphoglucose isomerase gene.

4. Requirement for phosphoglucose isomerase of Xanthomonas campestris in pathogenesis of citrus canker.

5. Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli.

6. A phosphoglucose isomerase gene is involved in the Rag phenotype of the yeast Kluyveromyces lactis.