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Literature DB >> 24271564

Engineering an isoprenoid pathway in Escherichia coli for production of 2-methyl-3-buten-2-ol: a potential biofuel.

Dinesh Gupta¹, Michael L Summers, Chhandak Basu.

Abstract

2-Methyl-3-buten-2-ol (MBO) is a natural volatile 5-carbon alcohol produced by several pine species that have the potential to be used as biofuel. MBO has a high energy content making it superior to ethanol in terms of energy output, and due to its volatility and lower solubility in water, MBO is easier to recover than ethanol. Pine's MBO synthase enzyme utilizes the intermediate dimethylallyl pyrophosphate (DMAPP) produced by the methyl-erythritol-4-phosphate isoprenoid pathway for the production of MBO. In this study, we performed metabolic engineering of Escherichia coli to express an alternate mevalonate dependent pathway for production of DMAPP, along with a codon optimized Pinus sabiniana MBO synthase gene. This heterologous expressed pathway carried out the conversion of an acetyl CoA precursor to DMAPP leading to production of MBO.

Entities: Chemical Species

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Year: 2014 PMID： 24271564 DOI： 10.1007/s12033-013-9721-1

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

21 in total

Review 1. The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants.

Authors: M Rohmer
Journal: Nat Prod Rep Date: 1999-10 Impact factor: 13.423

2. The production of the sesquiterpene β-caryophyllene in a transgenic strain of the cyanobacterium Synechocystis.

Authors: Robert E Reinsvold; Robert E Jinkerson; Randor Radakovits; Matthew C Posewitz; Chhandak Basu
Journal: J Plant Physiol Date: 2010-12-23 Impact factor: 3.549

Review 3. Biofuel alternatives to ethanol: pumping the microbial well.

Authors: J L Fortman; Swapnil Chhabra; Aindrila Mukhopadhyay; Howard Chou; Taek Soon Lee; Eric Steen; Jay D Keasling
Journal: Trends Biotechnol Date: 2008-05-28 Impact factor: 19.536

4. Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes.

Authors: B M Lange; T Rujan; W Martin; R Croteau
Journal: Proc Natl Acad Sci U S A Date: 2000-11-21 Impact factor: 11.205

5. Production of isoprenoid pharmaceuticals by engineered microbes.

Authors: Michelle C Y Chang; Jay D Keasling
Journal: Nat Chem Biol Date: 2006-12 Impact factor: 15.040

6. Biochemical characterization and homology modeling of methylbutenol synthase and implications for understanding hemiterpene synthase evolution in plants.

Authors: Dennis W Gray; Steven R Breneman; Lauren A Topper; Thomas D Sharkey
Journal: J Biol Chem Date: 2011-04-19 Impact factor: 5.157

7. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.

Authors: J Haseloff; K R Siemering; D C Prasher; S Hodge
Journal: Proc Natl Acad Sci U S A Date: 1997-03-18 Impact factor: 11.205

2. The Putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene.

Authors: Luca Rossoni; Stephen J Hall; Graham Eastham; Peter Licence; Gill Stephens
Journal: Appl Environ Microbiol Date: 2015-01-30 Impact factor: 4.792

2 in total

Engineering an isoprenoid pathway in Escherichia coli for production of 2-methyl-3-buten-2-ol: a potential biofuel.

Review 1. The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants.

2. The production of the sesquiterpene β-caryophyllene in a transgenic strain of the cyanobacterium Synechocystis.

Review 3. Biofuel alternatives to ethanol: pumping the microbial well.

4. Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes.

5. Production of isoprenoid pharmaceuticals by engineered microbes.

6. Biochemical characterization and homology modeling of methylbutenol synthase and implications for understanding hemiterpene synthase evolution in plants.

7. Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.

8. THE 1-DEOXY-D-XYLULOSE-5-PHOSPHATE PATHWAY OF ISOPRENOID BIOSYNTHESIS IN PLANTS.

9. Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.

10. Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli.

1. 2-Methyl-3-buten-2-ol (MBO) synthase expression in Nostoc punctiforme leads to over production of phytols.

2. The Putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene.