Specific transcription and reinitiation of class III genes in wheat embryo nuclei and chromatin.

Chromatin isolated from wheat germ embryos has a transcription efficiency for RNA polymerase III (pol III) closely approaching that for isolated wheat germ nuclei. Transcription in nuclei and chromatin is inhibited 5-10-fold by the addition of heparin, suggesting that free pol III molecules bind to chromatin and initiate transcription during thein vitro incubation. Nuclei were shown to have similar transcriptional activity in potassium chloride and potassium acetate. Nuclei and chromatin exhibited different salt optima for transcription. Neither nuclei nor chromatin were strongly stimulated by exogenous protein fractions. The data presented here suggest that in wheat germ nuclei the complete transcriptional apparatus is stably bound to the chromatin. Wheat germ nuclei may serve therefore as an enriched source for a solublein vitro transcription system.