| Literature DB >> 24269150 |
Miglena Manandhar1, John E Cronan2.
Abstract
Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) ligase (BioW), a biotin synthetic enzyme that converts pimelate, a seven-carbon dicarboxylic acid, to its CoA ester. The noncognate BioW substrate glutaric acid results in hydrolysis of ATP to AMP with formation of only trace amounts of glutaryl-CoA, thereby mimicking pretransfer editing of incorrect aminoacyl-adenylates by aminoacyl-tRNA synthetases.Entities:
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Year: 2013 PMID： 24269150 PMCID： PMC3927786 DOI： 10.1016/j.chembiol.2013.10.010
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521