Literature DB >> 2426296

An antigen-binding assay to determine the specificity of monoclonal antibodies against influenza virus and mapping of epitopes.

J A Lopez, M Guillen, A Sanchez-Fauquier, J A Melero.   

Abstract

A simple antigen-binding assay is described for determining the specificity of monoclonal antibodies against A/Victoria/3/75 influenza virus. Monoclonal antibodies were bound to polyvinylchloride microtitre wells via protein A and anti-immunoglobulin serum. Radiolabelled cell extracts were then added and allowed to adsorb to the antigen. The bound material was eluted and analyzed by polyacrylamide gel electrophoresis and autoradiography. The method can also be used to study competitive binding of antibodies to a given antigen. In this case, one antibody was adsorbed to the wells as above and the competitor antibody was added in high excess with the radioactive antigen. The results obtained with this method are comparable to those obtained by competitive RIA or ELISA.

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Year:  1986        PMID： 2426296     DOI： 10.1016/0166-0934(86)90019-4

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  12 in total

1.  Influenza virus matrix protein is the major driving force in virus budding.

Authors:  P Gómez-Puertas; C Albo; E Pérez-Pastrana; A Vivo; A Portela
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Genetic analysis of influenza virus NS1 gene: a temperature-sensitive mutant shows defective formation of virus particles.

Authors:  Urtzi Garaigorta; Ana M Falcón; Juan Ortín
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

3.  Conservation of epitopes recognized by monoclonal antibodies against the separated subunits of influenza hemagglutinin among type A viruses of the same and different subtypes.

Authors:  A Sanchez-Fauquier; M Guillen; J Martin; A P Kendal; J A Melero
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

4.  Analysis of genetic variability and mapping of point mutations in influenza virus by the RNase A mismatch cleavage method.

Authors:  C Lopez-Galindez; J A Lopez; J A Melero; L de la Fuente; C Martinez; J Ortin; M Perucho
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

5.  Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins.

Authors:  B García-Barreno; C Palomo; C Peñas; T Delgado; P Perez-Breña; J A Melero
Journal:  J Virol       Date:  1989-02       Impact factor: 5.103

6.  Phenotypic hiding: the carryover of mutations in RNA viruses as shown by detection of mar mutants in influenza virus.

Authors:  J Valcárcel; J Ortín
Journal:  J Virol       Date:  1989-09       Impact factor: 5.103

7.  Isolation of cross-reactive, subtype-specific monoclonal antibodies against influenza virus HA1 and HA2 hemagglutinin subunits.

Authors:  A Sánchez-Fauquier; N Villanueva; J A Melero
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

8.  Heterogeneity of the mutation rates of influenza A viruses: isolation of mutator mutants.

Authors:  P Suárez; J Valcárcel; J Ortín
Journal:  J Virol       Date:  1992-04       Impact factor: 5.103

9.  Serine 3 is critical for phosphorylation at the N-terminal end of the nucleoprotein of influenza virus A/Victoria/3/75.

Authors:  M Arrese; A Portela
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

10.  Mutational analysis of influenza A virus nucleoprotein: identification of mutations that affect RNA replication.

Authors:  I Mena; E Jambrina; C Albo; B Perales; J Ortín; M Arrese; D Vallejo; A Portela
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103
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