Literature DB >> 24259681

AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L.

Shoko Tsuboyama1, Yutaka Kodama.   

Abstract

The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). The procedure of the AgarTrap was carried out by simply exchanging appropriate solutions in a Petri dish, and completed within a week, successfully yielding sufficient numbers of independent transformants for molecular analysis (e.g. characterization of gene/protein function) in a single experiment. The AgarTrap method will promote future molecular biological study in M. polymorpha.

Entities:  

Keywords:  AgarTrap; Agrobacterium tumefaciens; Bryophytes; Genetic transformation; Marchantia polymorpha; Rhizobium radiobactor

Mesh：

Substances：

Year:  2013        PMID： 24259681     DOI： 10.1093/pcp/pct168

Source DB:  PubMed          Journal:  Plant Cell Physiol        ISSN： 0032-0781            Impact factor:   4.927


  17 in total

1.  A gain-of-function T-DNA insertion mutant of Marchantia polymorpha hyper-accumulates flavonoid riccionidin A.

Authors:  Noriko Hamashima; Xiaonan Xie; Mio Hikawa; Tomohiro Suzuki; Yutaka Kodama
Journal:  Plant Biotechnol (Tokyo)       Date:  2019-09-25       Impact factor: 1.133

2.  AgarTrap-mediated genetic transformation using intact gemmae/gemmalings of the liverwort Marchantia polymorpha L.

Authors:  Shoko Tsuboyama-Tanaka; Yutaka Kodama
Journal:  J Plant Res       Date:  2015-02-08       Impact factor: 2.629

3.  Phototropin perceives temperature based on the lifetime of its photoactivated state.

Authors:  Yuta Fujii; Hiroyuki Tanaka; Naotake Konno; Yuka Ogasawara; Noriko Hamashima; Saori Tamura; Satoshi Hasegawa; Yoshio Hayasaki; Koji Okajima; Yutaka Kodama
Journal:  Proc Natl Acad Sci U S A       Date:  2017-08-07       Impact factor: 11.205

Review 4.  Synthetic Botany.

Authors:  Christian R Boehm; Bernardo Pollak; Nuri Purswani; Nicola Patron; Jim Haseloff
Journal:  Cold Spring Harb Perspect Biol       Date:  2017-07-05       Impact factor: 10.005

Review 5.  Plant science's next top models.

Authors:  Igor Cesarino; Raffaele Dello Ioio; Gwendolyn K Kirschner; Michael S Ogden; Kelsey L Picard; Madlen I Rast-Somssich; Marc Somssich
Journal:  Ann Bot       Date:  2020-06-19       Impact factor: 4.357

6.  Highly efficient G-AgarTrap-mediated transformation of the Marchantia polymorpha model strains Tak-1 and Tak-2.

Authors:  Shoko Tsuboyama; Yutaka Kodama
Journal:  Plant Biotechnol (Tokyo)       Date:  2018-12-25       Impact factor: 1.133

7.  Actin-dependence of the chloroplast cold positioning response in the liverwort Marchantia polymorpha L.

Authors:  Shun Kimura; Yutaka Kodama
Journal:  PeerJ       Date:  2016-09-28       Impact factor: 2.984

8.  Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.

Authors:  Kimitsune Ishizaki; Ryuichi Nishihama; Minoru Ueda; Keisuke Inoue; Sakiko Ishida; Yoshiki Nishimura; Toshiharu Shikanai; Takayuki Kohchi
Journal:  PLoS One       Date:  2015-09-25       Impact factor: 3.240

9.  Time Gating of Chloroplast Autofluorescence Allows Clearer Fluorescence Imaging In Planta.

Authors:  Yutaka Kodama
Journal:  PLoS One       Date:  2016-03-30       Impact factor: 3.240

10.  DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha.

Authors:  Nagisa Nagaoka; Akihiro Yamashita; Rina Kurisu; Yuta Watari; Fumiko Ishizuna; Nobuhissro Tsutsumi; Kimitsune Ishizaki; Takayuki Kohchi; Shin-Ichi Arimura
Journal:  Sci Rep       Date:  2017-07-04       Impact factor: 4.379
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