Literature DB >> 24258375

Purification and properties of 5-enolpyruvylshikimate 3-phosphate synthase from seedlings of Pisum sativum L.

D M Mousdale1, J R Coggins.   

Abstract

5-Enolpyruvylshikimate 3-phosphate synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19) from shoot tissue of pea seedlings was purified to apparent homogeneity by sequential ammonium-sulphate precipitation, ion-exchange and hydrophobic-interaction chromatography and substrate elution from cellulose phosphate. Gel electrophoresis and gel-permeation chromatography showed that the purified enzyme was monomeric with molecular weight 50,000. The herbicide glyphosate was a potent inhibitor of the forward enzyme-catalyzed reaction.

Entities:  

Year:  1984        PMID： 24258375     DOI： 10.1007/BF00392469

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  22 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Purification and properties of the aromatic (arom) synthetic enzyme aggregate of Neurospora crassa.

Authors:  L Burgoyne; M E Case; N H Giles
Journal:  Biochim Biophys Acta       Date:  1969-11-04

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Enzymological basis for herbicidal action of glyphosate.

Authors:  J L Rubin; C G Gaines; R A Jensen
Journal:  Plant Physiol       Date:  1982-09       Impact factor: 8.340

5.  Purification and characterization of 3-dehydroquinate hydrolase and shikmate oxidoreductase. Evidence for a bifunctional enzyme.

Authors:  L D Polley
Journal:  Biochim Biophys Acta       Date:  1978-09-11

6.  The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.

Authors:  H C Steinrücken; N Amrhein
Journal:  Biochem Biophys Res Commun       Date:  1980-06-30       Impact factor: 3.575

7.  Dehydroquinate synthase in Bacillus subtilis. An enzyme associated with chorismate synthase and flavin reductase.

Authors:  N Hasan; E W Nester
Journal:  J Biol Chem       Date:  1978-07-25       Impact factor: 5.157

8.  Organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms.

Authors:  M B Berlyn; S I Ahmed; N H Giles
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490

9.  Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.

Authors:  M B Berlyn; N H Giles
Journal:  J Bacteriol       Date:  1969-07       Impact factor: 3.490

10.  Organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi.

Authors:  S I Ahmed; N H Giles
Journal:  J Bacteriol       Date:  1969-07       Impact factor: 3.490
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  7 in total

1.  Subcellular localization of the common shikimate-pathway enzymes in Pisum sativum L.

Authors:  D M Mousdale; J R Coggins
Journal:  Planta       Date:  1985-02       Impact factor: 4.116

2.  Expression of the hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate.

Authors:  A Peñaloza-Vázquez; A Oropeza; G L Mena; A M Bailey
Journal:  Plant Cell Rep       Date:  1995-05       Impact factor: 4.570

3.  Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis.

Authors:  H A Powell; N W Kerby; P Rowell; D M Mousdale; J R Coggins
Journal:  Planta       Date:  1992-11       Impact factor: 4.116

4.  Desensitizing plant EPSP synthase to glyphosate: Optimized global sequence context accommodates a glycine-to-alanine change in the active site.

Authors:  Yuxia Dong; Emily Ng; Jian Lu; Tamara Fenwick; Yumin Tao; Sean Bertain; Marian Sandoval; Ericka Bermudez; Zhenglin Hou; Phil Patten; Michael Lassner; Daniel Siehl
Journal:  J Biol Chem       Date:  2018-11-13       Impact factor: 5.157

5.  Overproduction by gene amplification of the multifunctional arom protein confers glyphosate tolerance to a plastid-free mutant of Euglena gracilis.

Authors:  S Reinbothe; B Ortel; B Parthier
Journal:  Mol Gen Genet       Date:  1993-06

6.  Mode of action of glyphosate in Candida maltosa.

Authors:  R Bode; C Melo; D Birnbaum
Journal:  Arch Microbiol       Date:  1984-11       Impact factor: 2.552

7.  Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate.

Authors:  Milosz Ruszkowski; Giuseppe Forlani
Journal:  Comput Struct Biotechnol J       Date:  2022-03-24       Impact factor: 7.271
  7 in total

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