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Literature DB >> 24178379

Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis.

H A Powell¹, N W Kerby, P Rowell, D M Mousdale, J R Coggins.

Abstract

5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.9) from the glyphosate-tolerant cyanobacterium Anabaena variabilis (ATCC 29413) was purified to homogeneity. The enzyme had a similar relative molecular mass to other EPSP synthases and showed similar kinetic properties except for a greatly elevated K i for the herbicide glyphosate (approximately ten times higher than that of enzymes from other sources). With whole cells, the monoisopropylamine salt of glyphosate was more toxic than the free acid but the effects of the free acid and monoisopropylamine salt on purified EPSP synthase were identical.

Entities: Chemical Species

Year: 1992 PMID： 24178379 DOI： 10.1007/BF00197039

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

36 in total

Purification and properties of a glyphosate-tolerant 5-enolpyruvylshikimate 3-phosphate synthase from the cyanobacterium Anabaena variabilis.

1. Cloning a gene coding for norflurazon resistance in cyanobacteria.

2. Subcellular localization of the common shikimate-pathway enzymes in Pisum sativum L.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. Codon usage in higher plants, green algae, and cyanobacteria.

5. An Altered aroA Gene Product Confers Resistance to the Herbicide Glyphosate.

6. Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase in a glyphosate-tolerant Petunia hybrida cell line.

7. Organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms.

8. Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.

9. Organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi.

10. Import of a precursor protein into chloroplasts is inhibited by the herbicide glyphosate.

1. Expression of the hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate.

2. Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro.