| Literature DB >> 24257624 |
Misty R Riddle1, Abraham Weintraub, Ken C Q Nguyen, David H Hall, Joel H Rothman.
Abstract
Terminally differentiated post-mitotic cells are generally considered irreversibly developmentally locked, i.e. incapable of being reprogrammed in vivo into entirely different cell types. We found that brief expression of a single transcription factor, the ELT-7 GATA factor, can convert the identity of fully differentiated, highly specialized non-endodermal cells of the pharynx into fully differentiated intestinal cells in intact larvae and adult Caenorhabditis elegans. Stable expression of intestine-specific molecular markers parallels loss of markers for the original differentiated pharynx state; hence, there is no apparent requirement for a dedifferentiated intermediate during the transdifferentiation process. Based on high-resolution morphological characteristics, the transdifferentiated cells become remodeled to resemble typical intestinal cells at the level of both the cell surface and internal organelles. Thus, post-mitotic cells, though terminally differentiated, remain plastic to transdifferentiation across germ layer lineage boundaries and can be remodeled to adopt the characteristics of a new cell identity without removal of inhibitory factors. Our findings establish a simple model to investigate how cell context influences forced transdifferentiation of mature cells.Entities:
Keywords: C. elegans; Cellular reprogramming; Transdifferentiation
Mesh:
Substances:
Year: 2013 PMID: 24257624 PMCID: PMC3848185 DOI: 10.1242/dev.103010
Source DB: PubMed Journal: Development ISSN: 0950-1991 Impact factor: 6.868