Literature DB >> 24257624

Transdifferentiation and remodeling of post-embryonic C. elegans cells by a single transcription factor.

Misty R Riddle1, Abraham Weintraub, Ken C Q Nguyen, David H Hall, Joel H Rothman.   

Abstract

Terminally differentiated post-mitotic cells are generally considered irreversibly developmentally locked, i.e. incapable of being reprogrammed in vivo into entirely different cell types. We found that brief expression of a single transcription factor, the ELT-7 GATA factor, can convert the identity of fully differentiated, highly specialized non-endodermal cells of the pharynx into fully differentiated intestinal cells in intact larvae and adult Caenorhabditis elegans. Stable expression of intestine-specific molecular markers parallels loss of markers for the original differentiated pharynx state; hence, there is no apparent requirement for a dedifferentiated intermediate during the transdifferentiation process. Based on high-resolution morphological characteristics, the transdifferentiated cells become remodeled to resemble typical intestinal cells at the level of both the cell surface and internal organelles. Thus, post-mitotic cells, though terminally differentiated, remain plastic to transdifferentiation across germ layer lineage boundaries and can be remodeled to adopt the characteristics of a new cell identity without removal of inhibitory factors. Our findings establish a simple model to investigate how cell context influences forced transdifferentiation of mature cells.

Entities:  

Keywords:  C. elegans; Cellular reprogramming; Transdifferentiation

Mesh:

Substances:

Year:  2013        PMID: 24257624      PMCID: PMC3848185          DOI: 10.1242/dev.103010

Source DB:  PubMed          Journal:  Development        ISSN: 0950-1991            Impact factor:   6.868


  28 in total

Review 1.  The C. elegans pharynx: a model for organogenesis.

Authors:  Susan E Mango
Journal:  WormBook       Date:  2007-01-22

Review 2.  Maintenance of C. elegans.

Authors:  Theresa Stiernagle
Journal:  WormBook       Date:  2006-02-11

3.  Reprogramming of early embryonic blastomeres into endodermal progenitors by a Caenorhabditis elegans GATA factor.

Authors:  J Zhu; T Fukushige; J D McGhee; J H Rothman
Journal:  Genes Dev       Date:  1998-12-15       Impact factor: 11.361

4.  pha-4, an HNF-3 homolog, specifies pharyngeal organ identity in Caenorhabditis elegans.

Authors:  M A Horner; S Quintin; M E Domeier; J Kimble; M Labouesse; S E Mango
Journal:  Genes Dev       Date:  1998-07-01       Impact factor: 11.361

5.  The myogenic potency of HLH-1 reveals wide-spread developmental plasticity in early C. elegans embryos.

Authors:  Tetsunari Fukushige; Michael Krause
Journal:  Development       Date:  2005-03-16       Impact factor: 6.868

6.  Temporal and spatial expression patterns of the small heat shock (hsp16) genes in transgenic Caenorhabditis elegans.

Authors:  E G Stringham; D K Dixon; D Jones; E P Candido
Journal:  Mol Biol Cell       Date:  1992-02       Impact factor: 4.138

7.  Direct visualization of the elt-2 gut-specific GATA factor binding to a target promoter inside the living Caenorhabditis elegans embryo.

Authors:  T Fukushige; M J Hendzel; D P Bazett-Jones; J D McGhee
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

8.  The GATA-factor elt-2 is essential for formation of the Caenorhabditis elegans intestine.

Authors:  T Fukushige; M G Hawkins; J D McGhee
Journal:  Dev Biol       Date:  1998-06-15       Impact factor: 3.582

9.  Translational regulators maintain totipotency in the Caenorhabditis elegans germline.

Authors:  Rafal Ciosk; Michael DePalma; James R Priess
Journal:  Science       Date:  2006-02-10       Impact factor: 47.728

10.  The Caenorhabditis elegans NK-2 class homeoprotein CEH-22 is involved in combinatorial activation of gene expression in pharyngeal muscle.

Authors:  P G Okkema; A Fire
Journal:  Development       Date:  1994-08       Impact factor: 6.868

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  15 in total

1.  Pluripotent cells will not dosage compensate.

Authors:  Jianhao Jiang; Alyssa C Lau; Györgyi Csankovszki
Journal:  Worm       Date:  2014-05-08

2.  Increasing Notch signaling antagonizes PRC2-mediated silencing to promote reprograming of germ cells into neurons.

Authors:  Stefanie Seelk; Irene Adrian-Kalchhauser; Balázs Hargitai; Martina Hajduskova; Silvia Gutnik; Baris Tursun; Rafal Ciosk
Journal:  Elife       Date:  2016-09-07       Impact factor: 8.140

3.  Quantitating transcription factor redundancy: The relative roles of the ELT-2 and ELT-7 GATA factors in the C. elegans endoderm.

Authors:  Aidan Dineen; Erin Osborne Nishimura; Barbara Goszczynski; Joel H Rothman; James D McGhee
Journal:  Dev Biol       Date:  2018-01-31       Impact factor: 3.582

Review 4.  Developmental Plasticity and Cellular Reprogramming in Caenorhabditis elegans.

Authors:  Joel Rothman; Sophie Jarriault
Journal:  Genetics       Date:  2019-11       Impact factor: 4.562

5.  Probing and rearranging the transcription factor network controlling the C. elegans endoderm.

Authors:  Tobias Wiesenfahrt; Erin Osborne Nishimura; Janette Y Berg; James D McGhee
Journal:  Worm       Date:  2016-06-10

Review 6.  Adult cell plasticity in vivo: de-differentiation and transdifferentiation are back in style.

Authors:  Allyson J Merrell; Ben Z Stanger
Journal:  Nat Rev Mol Cell Biol       Date:  2016-03-16       Impact factor: 94.444

Review 7.  Developmental genetics of the Caenorhabditis elegans pharynx.

Authors:  Marc Pilon
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2014-05-23       Impact factor: 5.814

8.  Regeneration of the digestive system in the crinoid Himerometra robustipinna occurs by transdifferentiation of neurosecretory-like cells.

Authors:  Nadezhda V Kalacheva; Marina G Eliseikina; Lidia T Frolova; Igor Yu Dolmatov
Journal:  PLoS One       Date:  2017-07-28       Impact factor: 3.240

9.  Coordinated control of terminal differentiation and restriction of cellular plasticity.

Authors:  Tulsi Patel; Oliver Hobert
Journal:  Elife       Date:  2017-04-19       Impact factor: 8.140

10.  Transorganogenesis and transdifferentiation in C. elegans are dependent on differentiated cell identity.

Authors:  Misty R Riddle; Erik A Spickard; Angela Jevince; Ken C Q Nguyen; David H Hall; Pradeep M Joshi; Joel H Rothman
Journal:  Dev Biol       Date:  2016-10-04       Impact factor: 3.582

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