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Literature DB >> 24248856

Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.

N Basiran¹, P Armitage, R J Scott, J Draper.

Abstract

Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.

Entities: Species

Year: 1987 PMID： 24248856 DOI： 10.1007/BF00269571

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

7 in total

Review 1. Nuclear dna amounts in angiosperms.

Authors: M D Bennett; J B Smith
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1976-05-27 Impact factor: 6.237

2. Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.

Authors: R J Scott; J Draper
Journal: Plant Mol Biol Date: 1987-05 Impact factor: 4.076

3. Nutrient requirements of suspension cultures of soybean root cells.

Authors: O L Gamborg; R A Miller; K Ojima
Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

4. Nopaline Ti-plasmid, pTiT37, T-DNA insertions into a flax genome.

Authors: A G Hepburn; L E Clarke; K S Blundy; J White
Journal: J Mol Appl Genet Date: 1983

5. Plant regeneration from protoplast-derived tissues of Linum usitatissimum L. (Flax).

Authors: M N Barakat; E C Cocking
Journal: Plant Cell Rep Date: 1983-12 Impact factor: 4.570

6. A Tissue-Culture Derived Salt-Tolerant Line of Flax (Linum usitatissimum).

Authors: A McHughen; M Swartz
Journal: J Plant Physiol Date: 2012-01-20 Impact factor: 3.549

7. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

Authors: P Zambryski; H Joos; C Genetello; J Leemans; M V Montagu; J Schell
Journal: EMBO J Date: 1983 Impact factor: 11.598

7 in total

12 in total

1. Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.

Authors: Sébastien Caillot; Emeline Rosiau; Catherine Laplace; Brigitte Thomasset
Journal: Plant Cell Rep Date: 2008-11-15 Impact factor: 4.570

2. Transformed callus does not necessarily regenerate transformed shoots.

Authors: M C Jordan; A McHughen
Journal: Plant Cell Rep Date: 1988-06 Impact factor: 4.570

3. A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n).

Authors: D Pental; A Mukhopadhyay; A Grover; A K Pradhan
Journal: Theor Appl Genet Date: 1988-08 Impact factor: 5.699

4. Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana.

Authors: R S Sangwan; Y Bourgeois; S Brown; G Vasseur; B Sangwan-Norreel
Journal: Planta Date: 1992-10 Impact factor: 4.116

Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.

Review 1. Nuclear dna amounts in angiosperms.

2. Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.

3. Nutrient requirements of suspension cultures of soybean root cells.

4. Nopaline Ti-plasmid, pTiT37, T-DNA insertions into a flax genome.

5. Plant regeneration from protoplast-derived tissues of Linum usitatissimum L. (Flax).

6. A Tissue-Culture Derived Salt-Tolerant Line of Flax (Linum usitatissimum).

7. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

1. Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.

2. Transformed callus does not necessarily regenerate transformed shoots.

3. A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n).

4. Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana.

5. Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L.).

6. ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens.

7. Improved flax regeneration from hypocotyls using thidiazuron as a cytokinin source.

8. High efficiency Agrobacterium-mediated gene transfer to flax.

9. Excision of the maize transposable element Ac in flax callus.

10. Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer.