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Literature DB >> 24241767

Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer.

Abstract

Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector containing a chimeric NPT-II gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. Transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. Co-segregation was observed in the progeny for kanamycin and glyphosate resistance.

Entities: Chemical Species

Year: 1988 PMID： 24241767 DOI： 10.1007/BF00272543

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

8 in total

1. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors.

Authors: J Fry; A Barnason; R B Horsch
Journal: Plant Cell Rep Date: 1987-10 Impact factor: 4.570

2. Nopaline Ti-plasmid, pTiT37, T-DNA insertions into a flax genome.

Authors: A G Hepburn; L E Clarke; K S Blundy; J White
Journal: J Mol Appl Genet Date: 1983

3. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.

Authors: H C Steinrücken; N Amrhein
Journal: Biochem Biophys Res Commun Date: 1980-06-30 Impact factor: 3.575

4. Engineering herbicide tolerance in transgenic plants.

Authors: D M Shah; R B Horsch; H J Klee; G M Kishore; J A Winter; N E Tumer; C M Hironaka; P R Sanders; C S Gasser; S Aykent; N R Siegel; S G Rogers; R T Fraley
Journal: Science Date: 1986-07-25 Impact factor: 47.728

5. A simple and general method for transferring genes into plants.

Authors:
Journal: Science Date: 1985-03-08 Impact factor: 47.728

6. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.

Authors: S McCormick; J Niedermeyer; J Fry; A Barnason; R Horsch; R Fraley
Journal: Plant Cell Rep Date: 1986-04 Impact factor: 4.570

7. Transformation of Medicago by Agrobacterium mediated gene transfer.

Authors: M Deak; G B Kiss; C Koncz; D Dudits
Journal: Plant Cell Rep Date: 1986-04 Impact factor: 4.570

8. Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.

Authors: N Basiran; P Armitage; R J Scott; J Draper
Journal: Plant Cell Rep Date: 1987-10 Impact factor: 4.570

8 in total

7 in total

Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer.

1. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors.

2. Nopaline Ti-plasmid, pTiT37, T-DNA insertions into a flax genome.

3. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.

4. Engineering herbicide tolerance in transgenic plants.

5. A simple and general method for transferring genes into plants.

6. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.

7. Transformation of Medicago by Agrobacterium mediated gene transfer.

8. Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.

1. Recovery of transgenic plants from "escape" shoots.

2. ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens.

3. Agrobacterium mediated transfer of chlorsulfuron resistance to commercial flax cultivars.

4. Improved flax regeneration from hypocotyls using thidiazuron as a cytokinin source.

5. High efficiency Agrobacterium-mediated gene transfer to flax.

6. Patterns of transformation intensity on flax hypocotyls inoculated with Agrobacterium tumefaciens.

7. Establishment of efficient regeneration protocol for three rapeseed cultivars.