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Literature DB >> 24248653

Transformation of Stylosanthes spp. using Agrobacterium tumefaciens.

Abstract

Tumours were incited on leaf sections of Stylosanthes humilis, S. hamata, S. guianensis and S. scalra following infection by Agrobacterium tumefaciens. The suitability of 2 binary vectors (pGA472, BIN6) for gene transfer in S. humilis was tested and kanamycin-resistant tumour tissue was obtained from infected leaf pieces. The presence and expression of the neomycin phosphotransferase (NPT II) gene in the plant cells was demonstrated by hybridization of the coding region of the NPT II gene of the transposon Tn5 to DNA and RNA of kanamycin resistant tumours and by detection of significant NPT II activity in tissue extracts. Tumours also produced teratomatous shoots expressing the NPT II gene, but these could not be rooted.

Entities: Chemical Disease Species

Year: 1987 PMID： 24248653 DOI： 10.1007/BF00268480

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

10 in total

Review 1. The molecular genetics of crown gall tumorigenesis.

Authors: P J Hooykaas; R A Schilperoort
Journal: Adv Genet Date: 1984 Impact factor: 1.944

2. The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature.

Authors: R H Hamilton; M Z Fall
Journal: Experientia Date: 1971-02-15

3. Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traits.

Authors: F Yang; A L Montoya; D J Merlo; M H Drummond; M D Chilton; E W Nester; M P Gordon
Journal: Mol Gen Genet Date: 1980

4. Decrease in Three Messenger RNA Species Coding for Chloroplast Proteins in Leaves of Barley Infected with Erysiphe graminis f. sp. hordei.

Authors: C M Higgins; J M Manners; K J Scott
Journal: Plant Physiol Date: 1985-08 Impact factor: 8.340

5. Binary Agrobacterium vectors for plant transformation.

Authors: M Bevan
Journal: Nucleic Acids Res Date: 1984-11-26 Impact factor: 16.971

6. A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources.

Authors: D P Weeks; N Beerman; O M Griffith
Journal: Anal Biochem Date: 1986-02-01 Impact factor: 3.365

7. Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions.

Authors: G Ooms; P J Hooykaas; G Moolenaar; R A Schilperoort
Journal: Gene Date: 1981 Jun-Jul Impact factor: 3.688

8. Transformation of forage legumes using Agrobacterium tumefaciens.

Authors: K J Webb
Journal: Theor Appl Genet Date: 1986-04 Impact factor: 5.699

9. The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts.

Authors: P H Schreier; E A Seftor; J Schell; H J Bohnert
Journal: EMBO J Date: 1985-01 Impact factor: 11.598

10. New cloning vehicles for transformation of higher plants.

Authors: G An; B D Watson; S Stachel; M P Gordon; E W Nester
Journal: EMBO J Date: 1985-02 Impact factor: 11.598

10 in total

5 in total

1. Efficient transformation with regeneration of the tropical pasture legumeStylosanthes humilis usingAgrobacterium rhizogenes and a Ti plasmid-binary vector system.

Authors: J M Manners; H Way
Journal: Plant Cell Rep Date: 1989-06 Impact factor: 4.570

5. Transgenic white clover. Studies with the auxin-responsive promoter, GH3, in root gravitropism and lateral root development.

Authors: P J Larkin; J M Gibson; U Mathesius; J J Weinman; E Gartner; E Hall; G J Tanner; B G Rolfe; M A Djordjevic
Journal: Transgenic Res Date: 1996-09 Impact factor: 2.788