| Literature DB >> 24241599 |
Abstract
Large yields (1.85 × 10(7)/g.f.wt.) of viable protoplasts were obtained from leaves of axenic shoot cultures of Malus Xdomestica Borkh. cv. 'Greensleeves'. Protoplasts cultured in liquid or agarose semi-solidified KM8P medium underwent cell wall regeneration and colony formation.Protoplast-derived cell colonies developed to callus on semi-solid KM8 medium. This is the first report of callus formation from mesophyll protoplasts of apple.Entities:
Year: 1988 PMID: 24241599 DOI: 10.1007/BF00269323
Source DB: PubMed Journal: Plant Cell Rep ISSN: 0721-7714 Impact factor: 4.570