Literature DB >> 24241542

Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants.

D Catlin1, O Ochoa, S McCormick, C F Quiros.   

Abstract

Transgenic celery plants were obtained following co-cultivation of petiole explants with Agrobacterlum tumefaciens containing pMON200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. Transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. Transformation was confirmed either by the presence of nopaline or by Southern blots. Cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and number. Only 20% of the regenerated plants had the normal karyotype. Kanamycin resistance behaved as a monogenic, dominant trait, segregating in a 3:1 ratio in three families derived from plants with normal karyotypes.

Entities:  

Year:  1988        PMID： 24241542     DOI： 10.1007/BF00270114

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  4 in total

1.  A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Authors:  L A Otten; R A Schilperoort
Journal:  Biochim Biophys Acta       Date:  1978-12-08

2.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

3.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

4.  Expression of bacterial genes in plant cells.

Authors:  R T Fraley; S G Rogers; R B Horsch; P R Sanders; J S Flick; S P Adams; M L Bittner; L A Brand; C L Fink; J S Fry; G R Galluppi; S B Goldberg; N L Hoffmann; S C Woo
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205
  4 in total
  7 in total

1.  Identification of a mannitol transporter, AgMaT1, in celery phloem.

Authors:  N Noiraud; L Maurousset; R Lemoine
Journal:  Plant Cell       Date:  2001-03       Impact factor: 11.277

2.  Plant regeneration from sugarbeet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants.

Authors:  B Jacq; T Tétu; R S Sangwan; A De Laat; B S Sangwan-Norreel
Journal:  Plant Cell Rep       Date:  1992-07       Impact factor: 4.570

Review 3.  Transformation of plant cells via Agrobacterium.

Authors:  P J Hooykaas
Journal:  Plant Mol Biol       Date:  1989-09       Impact factor: 4.076

4.  In vitro regeneration of wild chervil (Anthriscus sylvestris L.).

Authors:  Oktavia Hendrawati; Jacques Hille; Herman J Woerdenbag; Wim J Quax; Oliver Kayser
Journal:  In Vitro Cell Dev Biol Plant       Date:  2011-10-25       Impact factor: 2.252

5.  Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens.

Authors:  C N Liu; X Q Li; S B Gelvin
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

6.  Effect of culture conditions on Agrobacterium-mediated transformation in datura.

Authors:  R S Sangwan; C Ducrocq; B S Sangwan-Norreel
Journal:  Plant Cell Rep       Date:  1991-06       Impact factor: 4.570

7.  Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants.

Authors:  G Fang; R Grumet
Journal:  Plant Cell Rep       Date:  1990-07       Impact factor: 4.570
  7 in total

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