Literature DB >> 24240368

Purification and properties of ascorbate free-radical reductase from potato tubers.

G Borraccino1, S Dipierro, O Arrigoni.   

Abstract

Ascrobate free-radical reductase (EC 1.6.5.4) from potato tubers was purified to apparent homogencity by a method which included ammonium-sulfate precipitation, gel filtration and chromatography on diethylaminoethyl cellulose and hydroxylapatite. Gel filtration and gel electrophoresis showed that the purified enzyme was monomeric with a molecular weight of about 42 000. Enzyme activity was heat lable and severely inhibited by thiol reagents. The Km values for enzyme substrates were estimated.

Entities:  

Year:  1986        PMID： 24240368     DOI： 10.1007/BF00391228

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  11 in total

1.  [The mechanism of action of ascorbic acid. I. Isolation of an ascorbic acid-dependent dihydrodiphosphopyridine nucleotide oxidase from the adrenal microsomes].

Authors:  H KERSTEN; W KERSTEN; H STAUDINGER
Journal:  Biochim Biophys Acta       Date:  1958-03

2.  The Oxidation of Reduced Diphosphopyridine Nucleotide by an Ascorbate System from Cucumber.

Authors:  H Beevers
Journal:  Plant Physiol       Date:  1954-05       Impact factor: 8.340

3.  Activation of a DPNH oxidase by an oxidation product of ascorbic acid.

Authors:  M KERN; E RACKER
Journal:  Arch Biochem Biophys       Date:  1954-01       Impact factor: 4.013

4.  The enzymatic oxidation of reduced pyridine nucleotides by an oxidation product of ascorbic acid.

Authors:  A NASON; W D WOSILAIT; A J TERRELL
Journal:  Arch Biochem Biophys       Date:  1954-01       Impact factor: 4.013

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  [The isolation and characterization of a NADH: semidehydroascorbic acid oxidoreductase from Neurospora crassa].

Authors:  H U Schulze; H H Schott; H Staudinger
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1972-12

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Oxidation of ascorbate anion by electron transfer to phenoxyl radicals.

Authors:  R H Schuler
Journal:  Radiat Res       Date:  1977-03       Impact factor: 2.841

9.  The involvement of semidehydroascorbate reductase in the oxidation of NADH by lipid peroxide in mitochondria and microsomes.

Authors:  R C Green; P J O'Brien
Journal:  Biochim Biophys Acta       Date:  1973-02-15

10.  Participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in NADH-semidehydroascorbic acid reductase activity of rat liver.

Authors:  A Ito; S Hayashi; T Yoshida
Journal:  Biochem Biophys Res Commun       Date:  1981-07-30       Impact factor: 3.575
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  9 in total

1.  Dissecting the superoxide dismutase-ascorbate-glutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis.

Authors:  A Polle
Journal:  Plant Physiol       Date:  2001-05       Impact factor: 8.340

Review 2.  Metal/metalloid stress tolerance in plants: role of ascorbate, its redox couple, and associated enzymes.

Authors:  Naser A Anjum; Sarvajeet S Gill; Ritu Gill; Mirza Hasanuzzaman; Armando C Duarte; Eduarda Pereira; Iqbal Ahmad; Renu Tuteja; Narendra Tuteja
Journal:  Protoplasma       Date:  2014-03-29       Impact factor: 3.356

3.  Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress.

Authors:  Sun-Young Shin; Myung-Hee Kim; Yul-Ho Kim; Hyang-Mi Park; Ho-Sung Yoon
Journal:  Mol Cells       Date:  2013-10-22       Impact factor: 5.034

4.  Overexpression of monodehydroascorbate reductase in transgenic tobacco confers enhanced tolerance to ozone, salt and polyethylene glycol stresses.

Authors:  Amin Elsadig Eltayeb; Naoyoshi Kawano; Ghazi Hamid Badawi; Hironori Kaminaka; Takeshi Sanekata; Toshiyuki Shibahara; Shinobu Inanaga; Kiyoshi Tanaka
Journal:  Planta       Date:  2006-10-17       Impact factor: 4.116

5.  NADH-Monodehydroascorbate oxidoreductase is one of the redox enzymes in spinach leaf plasma membranes

Authors: 
Journal:  Plant Physiol       Date:  1998-03       Impact factor: 8.340

Review 6.  Ascorbate and plant cell growth.

Authors:  F Córdoba; J A González-Reyes
Journal:  J Bioenerg Biomembr       Date:  1994-08       Impact factor: 2.945

7.  Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.

Authors:  Geert Potters; Nele Horemans; Silvia Bellone; Roland J Caubergs; Paolo Trost; Yves Guisez; Han Asard
Journal:  Plant Physiol       Date:  2004-03-26       Impact factor: 8.340

8.  Genome-Wide Analysis of MDHAR Gene Family in Four Cotton Species Provides Insights into Fiber Development via Regulating AsA Redox Homeostasis.

Authors:  Fangfang Zhou; Bowen Zheng; Fei Wang; Aiping Cao; Shuangquan Xie; Xifeng Chen; Joel A Schick; Xiang Jin; Hongbin Li
Journal:  Plants (Basel)       Date:  2021-01-25

9.  Zebra chip disease enhances respiration and oxidative stress of potato tubers (Solanum tuberosum L.).

Authors:  G N Mohan Kumar; Lisa O Knowles; N Richard Knowles
Journal:  Planta       Date:  2017-06-16       Impact factor: 4.116
  9 in total

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