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Literature DB >> 2423529

Probing the structure of cytoplasm.

Abstract

We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 cells by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 A, but may be as small as 200 A in some cytoplasmic domains.

Entities: CellLine Chemical

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Year: 1986 PMID： 2423529 PMCID： PMC2114258 DOI： 10.1083/jcb.102.6.2015

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

38 in total

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Authors: D Bray; C Thomas
Journal: Biochem J Date: 1975-05 Impact factor: 3.857

2. ON THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. II. THE TRANSPORT OF GLOBULAR PARTICLES THROUGH HYALURONIC ACID SOLUTIONS.

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Journal: Biochim Biophys Acta Date: 1963-10-29

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Authors: M A LAUFFER
Journal: Biophys J Date: 1961-01 Impact factor: 4.033

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Authors: G GROTTE
Journal: Acta Chir Scand Suppl Date: 1956

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Authors: D L Taylor; J S Condeelis
Journal: Int Rev Cytol Date: 1979

6. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.

Authors: P J Sims; A S Waggoner; C H Wang; J F Hoffman
Journal: Biochemistry Date: 1974-07-30 Impact factor: 3.162

7. Studies on the length of the side chains of the dextran elaborated by Leuconostoc mesenteroides NRRL B-512.

Authors: O Larm; B Lindberg; S Svensson
Journal: Carbohydr Res Date: 1971-11 Impact factor: 2.104

8. Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

Authors: I Blikstad; F Markey; L Carlsson; T Persson; U Lindberg
Journal: Cell Date: 1978-11 Impact factor: 41.582

9. Purification of the 300K intermediate filament-associated protein and its in vitro recombination with intermediate filaments.

Authors: N Lieska; H Y Yang; R D Goldman
Journal: J Cell Biol Date: 1985-09 Impact factor: 10.539

9. The Use of Fluorescent Tracers to Characterize the Post-Phloem Transport Pathway in Maternal Tissues of Developing Wheat Grains.

Authors: N. Wang; D. B. Fisher
Journal: Plant Physiol Date: 1994-01 Impact factor: 8.340

10. Fluid displacement during droplet formation at microfluidic flow-focusing junctions.

Authors: Haishui Huang; Xiaoming He
Journal: Lab Chip Date: 2015-09-18 Impact factor: 6.799

Probing the structure of cytoplasm.

1. The actin content of fibroblasts.

2. ON THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. II. THE TRANSPORT OF GLOBULAR PARTICLES THROUGH HYALURONIC ACID SOLUTIONS.

3. Theory of diffusion in gels.

4. Passage of dextran molecules across the blood-lymph barrier.

Review 5. Cytoplasmic structure and contractility in amoeboid cells.

6. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles.

7. Studies on the length of the side chains of the dextran elaborated by Leuconostoc mesenteroides NRRL B-512.

8. Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

9. Purification of the 300K intermediate filament-associated protein and its in vitro recombination with intermediate filaments.

10. Behavior of a fluorescent analogue of calmodulin in living 3T3 cells.

1. Neuropeptide release by efficient recruitment of diffusing cytoplasmic secretory vesicles.

2. Mechanics of living cells measured by laser tracking microrheology.

3. Spatial buffering of potassium ions in brain extracellular space.

4. Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery.

5. A new model for nuclear envelope breakdown.

6. Dissipative metabolic patterns respond during neutrophil transmembrane signaling.

7. Tracking single proteins within cells.

8. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

9. The Use of Fluorescent Tracers to Characterize the Post-Phloem Transport Pathway in Maternal Tissues of Developing Wheat Grains.

10. Fluid displacement during droplet formation at microfluidic flow-focusing junctions.