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Literature DB >> 24232983

Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens.

Abstract

Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.

Entities: Chemical Disease Gene

Year: 1989 PMID： 24232983 DOI： 10.1007/BF00716837

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

12 in total

1. A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Authors: L A Otten; R A Schilperoort
Journal: Biochim Biophys Acta Date: 1978-12-08

2. The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature.

Authors: R H Hamilton; M Z Fall
Journal: Experientia Date: 1971-02-15

3. The functional organization of the nopaline A. tumefaciens plasmid pTiC58.

Authors: M Holsters; B Silva; F Van Vliet; C Genetello; M De Block; P Dhaese; A Depicker; D Inzé; G Engler; R Villarroel
Journal: Plasmid Date: 1980-03 Impact factor: 3.466

4. The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6s3.

Authors: H De Greve; H Decraemer; J Seurinck; M Van Montagu; J Schell
Journal: Plasmid Date: 1981-09 Impact factor: 3.466

5. Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors: J Velten; J Schell
Journal: Nucleic Acids Res Date: 1985-10-11 Impact factor: 16.971

6. Genetic analysis of T-DNA transcripts in nopaline crown galls.

Authors: H Joos; D Inzé; A Caplan; M Sormann; M Van Montagu; J Schell
Journal: Cell Date: 1983-04 Impact factor: 41.582

7. Shoot regeneration of mesophyll protoplasts transformed by Agrobacterium tumefaciens, not achievable with untransformed protoplasts.

Authors: A Steffen; T Eriksson; O Schieder
Journal: Theor Appl Genet Date: 1986-04 Impact factor: 5.699

8. Genetic transformation of 9 in vitro clones of Alnus and Betula by Agrobacterium tumefaciens.

Authors: J Mackay; A Séguin; M Lalonde
Journal: Plant Cell Rep Date: 1988-06 Impact factor: 4.570

9. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

Authors: P Zambryski; H Joos; C Genetello; J Leemans; M V Montagu; J Schell
Journal: EMBO J Date: 1983 Impact factor: 11.598

10. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.

Authors: J Leemans; R Deblaere; L Willmitzer; H De Greve; J P Hernalsteens; M Van Montagu; J Schell
Journal: EMBO J Date: 1982 Impact factor: 11.598

7 in total

1. An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.

Authors: A C Brasileiro; J C Leplé; J Muzzin; D Ounnoughi; M F Michel; L Jouanin
Journal: Plant Mol Biol Date: 1991-09 Impact factor: 4.076

2. The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

Authors: I Godwin; G Todd; B Ford-Lloyd; H J Newbury
Journal: Plant Cell Rep Date: 1991-04 Impact factor: 4.570

3. Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains.

Authors: M M Lulsdorf; H Rempel; J A Jackson; D S Baliski; S L Hobbs
Journal: Plant Cell Rep Date: 1991-01 Impact factor: 4.570

7. Hairy Root Transformation: A Useful Tool to Explore Gene Function and Expression in Salix spp. Recalcitrant to Transformation.

Authors: Carolina Gomes; Annabelle Dupas; Andrea Pagano; Jacqueline Grima-Pettenati; Jorge Almiro P Paiva
Journal: Front Plant Sci Date: 2019-11-11 Impact factor: 5.753