Literature DB >> 24232780

Genetic transformation of Medicago species by Agrobacterium tumefaciens and electroporation of protoplasts.

N Kuchuk1, I Komarnitski, A Shakhovsky, Y Gleba.   

Abstract

Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA in to the genome of the regenerated plants.Transgenic plants resistant to kanamycin were obtained by electroporation of Medicago borealis protoplasts with the pGA 472 plasmid DNA.

Entities:  

Year:  1990        PMID： 24232780     DOI： 10.1007/BF00269987

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  16 in total

1.  Identification of a cloned cytokinin biosynthetic gene.

Authors:  G F Barry; S G Rogers; R T Fraley; L Brand
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

2.  A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Authors:  L A Otten; R A Schilperoort
Journal:  Biochim Biophys Acta       Date:  1978-12-08

3.  Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media.

Authors:  K N Kao; M R Michayluk
Journal:  Planta       Date:  1975-01       Impact factor: 4.116

4.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

5.  Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.

Authors:  J Leemans; C Shaw; R Deblaere; H De Greve; J P Hernalsteens; M Maes; M Van Montagu; J Schell
Journal:  J Mol Appl Genet       Date:  1981

6.  T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.

Authors:  D E Akiyoshi; H Klee; R M Amasino; E W Nester; M P Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

7.  Hairy root transformation in alfalfa (Medicago sativa L.).

Authors:  L Spanò; D Mariotti; M Pezzotti; F Damiani; S Arcioni
Journal:  Theor Appl Genet       Date:  1987-02       Impact factor: 5.699

8.  Transformation of Medicago by Agrobacterium mediated gene transfer.

Authors:  M Deak; G B Kiss; C Koncz; D Dudits
Journal:  Plant Cell Rep       Date:  1986-04       Impact factor: 4.570

9.  Stable transformation of moth bean Vigna aconitifolia via direct gene transfer.

Authors:  F Köhler; C Golz; S Eapen; H Kohn; O Schieder
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

10.  Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco.

Authors:  J Memelink; J H Hoge; R A Schilperoort
Journal:  EMBO J       Date:  1987-12-01       Impact factor: 11.598
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