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Literature DB >> 24232344

Structure of melon rDNA and nucleotide sequence of the 17-25S spacer region.

Abstract

Restriction enzyme and hybridization analysis of melon nuclear DNA suggests a homogenous rDNA population with a repeat unit of 10.2 kb. Several full length Hind III rDNA repeat units were cloned and one of these is described in detail. The regions coding for 25S, 17S and 5.8S rRNAs were located by crossed-contact hybridization and R-loop mapping. Introns were not observed. The nucleotide sequence of the internal transcribed spacer and flanking regions was determined and compared with the corresponding region from rice rDNA by dot matrix analysis. In addition, the extent of gross sequence homology between cloned melon and pea rDNA units was determined by heteroduplex mapping.

Entities: Species

Year: 1988 PMID： 24232344 DOI： 10.1007/BF00303512

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

34 in total

1. An improved method for detecting foreign DNA in plasmids of Escherichia coli.

Authors: R E Thayer
Journal: Anal Biochem Date: 1979-09-15 Impact factor: 3.365

2. The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

Authors: Y Urano; R Kominami; Y Mishima; M Muramatsu
Journal: Nucleic Acids Res Date: 1980-12-20 Impact factor: 16.971

3. The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.

Authors: T Moss; M L Birnstiel
Journal: Nucleic Acids Res Date: 1979-08-24 Impact factor: 16.971

4. Cloning and characterization of ribosomal RNA genes from wheat and barley.

Authors: W L Gerlach; J R Bedbrook
Journal: Nucleic Acids Res Date: 1979-12-11 Impact factor: 16.971

5. Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.

Authors: E S Coen; G A Dover
Journal: Nucleic Acids Res Date: 1982-11-11 Impact factor: 16.971

6. Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.

Authors: M P Verbeet; J Klootwijk; H van Heerikhuizen; R Fontijn; E Vreugdenhil; R J Planta
Journal: Gene Date: 1983-07 Impact factor: 3.688

7. The genes for 18S, 5.8S and 28S ribosomal RNA of Bombyx mori are organized into tandem repeats of uniform length.

Authors: R F Manning; D R Samols; L P Gage
Journal: Gene Date: 1978-10 Impact factor: 3.688

8. Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.

Authors: Y Mishima; I Financsek; R Kominami; M Muramatsu
Journal: Nucleic Acids Res Date: 1982-11-11 Impact factor: 16.971

9. Characterisation of the genes for ribosomal RNA in flax.

Authors: P B Goldsbrough; C A Cullis
Journal: Nucleic Acids Res Date: 1981-03-25 Impact factor: 16.971

10. Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.

Authors: E O Long; M L Rebbert; I B Dawid
Journal: Proc Natl Acad Sci U S A Date: 1981-03 Impact factor: 11.205

6 in total

Structure of melon rDNA and nucleotide sequence of the 17-25S spacer region.

1. An improved method for detecting foreign DNA in plasmids of Escherichia coli.

2. The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

3. The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.

4. Cloning and characterization of ribosomal RNA genes from wheat and barley.

5. Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.

6. Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.

7. The genes for 18S, 5.8S and 28S ribosomal RNA of Bombyx mori are organized into tandem repeats of uniform length.

8. Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.

9. Characterisation of the genes for ribosomal RNA in flax.

10. Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.

1. Nucleotide sequence of a 5.8S rDNA gene and of the internal transcribed spacers from Populus deltoides.

2. Molecular evidence for transcription of genes on a B chromosome in Crepis capillaris.

3. GC balance in the internal transcribed spacers ITS 1 and ITS 2 of nuclear ribosomal RNA genes.

4. Paternal inheritance of mitochondria and chloroplasts in Festuca pratensis-Lolium perenne intergeneric hybrids.

5. A conserved sequence in internal transcribed spacer 1 of plant nuclear rRNA genes.

6. Angiosperm origin and early stages of seed plant evolution deduced from rRNA sequence comparisons.