Literature DB >> 24231996

Soybean 11S globulin polypeptides have an antigenic homology with 11S globulins from various plants.

A Y Alexenko1, I V Nikolaev, Y P Vinetski.   

Abstract

Rabbit serum antibodies (AB) against glycinin acidic polypeptides were separated by cross exhausting, and the antibody fractions for each of the two subfamilies of glycinin subunits (A1 and A3) were obtained. The antibodies were used in the immuno blot assay with seed protein of various plant classes. Polypeptides homologous to soybean glycinin were detected. Homology with A1 polypeptide was revealed in more cases than with A3. Total seed protein preparations were subjected to centrifugation in sucrose density gradient, and the polypeptides, imunochemically related to glycinin, occurred only in fractions with sedimentation constant about 11S. The nativity of conservative antigenic determinants of 11S globulins is discussed.

Entities:  

Year:  1988        PMID: 24231996     DOI: 10.1007/BF00288845

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN: 0040-5752            Impact factor:   5.699


  11 in total

1.  Seed globulins of the Gramineae and Leguminosae.

Authors:  C E Danielsson
Journal:  Biochem J       Date:  1949       Impact factor: 3.857

2.  Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) : I. Legumin.

Authors:  R R Croy; J A Gatehouse; I M Evans; D Boulter
Journal:  Planta       Date:  1980-02       Impact factor: 4.116

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds.

Authors:  R Casey
Journal:  Biochem J       Date:  1979-02-01       Impact factor: 3.857

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The biosynthesis and processing of high molecular weight precursors of soybean glycinin subunits.

Authors:  K A Barton; J F Thompson; J T Madison; R Rosenthal; N P Jarvis; R N Beachy
Journal:  J Biol Chem       Date:  1982-06-10       Impact factor: 5.157

7.  Comparison of the antigenic properties of the glycinin polypeptides.

Authors:  M A Moreira; W C Mahoney; B A Larkins; N C Nielsen
Journal:  Arch Biochem Biophys       Date:  1981-09       Impact factor: 4.013

8.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors:  J J Sedmak; S E Grossberg
Journal:  Anal Biochem       Date:  1977-05-01       Impact factor: 3.365

9.  Proteins of soybean seeds: I. Isolation and characterization of the major components.

Authors:  J E Hill; R W Breidenbach
Journal:  Plant Physiol       Date:  1974-05       Impact factor: 8.340

10.  The globulin seed storage proteins of flowering plants are derived from two ancestral genes.

Authors:  K Borroto; L Dure
Journal:  Plant Mol Biol       Date:  1987-03       Impact factor: 4.076

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  1 in total

1.  An attempt to elucidate the origin of cultivated soybean via comparison of nucleotide sequences encoding glycinin B4 polypeptide of cultivated soybean, Glycine max, and its presumed wild progenitor, Glycine soja.

Authors:  E S Zakharova; S M Epishin; Y P Vinetski
Journal:  Theor Appl Genet       Date:  1989-12       Impact factor: 5.699

  1 in total

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