Literature DB >> 24227166

Somatic hybrid plants produced by electrofusion between dihaploid potatoes: BF15 (H1), Aminca (H6) and Cardinal (H3).

M H Chaput1, D Sihachakr, G Ducreux, D Marie, N Barghi.   

Abstract

In order to regenerate somatic hybrids, mesophyll protoplasts from a dihaploid potato, BF15 (H1), were electrofused with those from two other dihaploid clones, Aminca (H6) and Cardinal (H3). Determination of the ploidy level by flow cytometry showed that 10% of plants regenerated from the fusion experiment with "BF15 + Aminca" were diploids, 14% triploids, 63% tetraploids and very few were mixoploids or had a higher ploidy level. Using morphological markers and vigour in plant growth, we were able to recover a total of 24 somatic hybrid plants, respectively 20 and 4 hybrids (accounting for 12% and 13% of regenerants) from the fusions "BF15 + Aminca" and "BF15 + Cardinal". Most of the somatic hybrids were at the expected tetraploid level (2n=4x=48). The hybrid nature was confirmed by examining isoenzyme patterns for malate dehydrogenase (MDH) and isocitrate dehydrogenase (ICD).

Entities:  

Year:  1990        PMID： 24227166     DOI： 10.1007/BF00232261

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  8 in total

1.  Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae.

Authors:  J Fahleson; J Dixelius; E Sundberg; K Glimelius
Journal:  Plant Cell Rep       Date:  1988-01       Impact factor: 4.570

2.  Production of somatic hybrids by electrofusion in Solanum.

Authors:  N Fish; A Karp; M G Jones
Journal:  Theor Appl Genet       Date:  1988-08       Impact factor: 5.699

3.  The isolation, culture and regeneration of Petunia leaf protoplasts.

Authors:  E M Frearson; J B Power; E C Cocking
Journal:  Dev Biol       Date:  1973-07       Impact factor: 3.582

4.  Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis.

Authors:  S Waara; H Tegelström; A Wallin; T Eriksson
Journal:  Theor Appl Genet       Date:  1989-01       Impact factor: 5.699

5.  Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw.

Authors:  D Sihachakr; R Haicour; M H Chaput; E Barrientos; G Ducreux; L Rossignol
Journal:  Theor Appl Genet       Date:  1989-01       Impact factor: 5.699

6.  Selection of somatic hybrids between diploid clones of potato (Solanum tuberosum L.) transformed by direct gene transfer.

Authors:  J Masson; D Lancelin; C Bellini; M Lecerf; P Guerche; G Pelletier
Journal:  Theor Appl Genet       Date:  1989-08       Impact factor: 5.699

7.  Intra-specific fusions in Solanum tuberosum.

Authors:  S Austin; M Baer; M Ehlenfeldt; P J Kazmierczak; J P Helgeson
Journal:  Theor Appl Genet       Date:  1985-12       Impact factor: 5.699

8.  Somatic hybrid potato plants after electrofusion of diploid Solanum tuberosum and Solanum phureja.

Authors:  K J Puite; S Roest; L P Pijnacker
Journal:  Plant Cell Rep       Date:  1986-08       Impact factor: 4.570
  8 in total
  14 in total

1.  Molecular characterisation of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers.

Authors:  E Baird; S Cooper-Bland; R Waugh; M DeMaine; W Powell
Journal:  Mol Gen Genet       Date:  1992-06

2.  Field evaluation of tetraploid somatic potato hybrids.

Authors:  C Möllers; U Frei; G Wenzel
Journal:  Theor Appl Genet       Date:  1994-05       Impact factor: 5.699

3.  Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens.

Authors:  Y S Xu; M S Clark; E Pehu
Journal:  Plant Cell Rep       Date:  1993-01       Impact factor: 4.570

4.  Interaction between cytoplasmic composition and yield parameters in somatic hybrids of S. tuberosum L.

Authors:  A Lössl; U Frei; G Wenzel
Journal:  Theor Appl Genet       Date:  1994-12       Impact factor: 5.699

5.  Tetraploid potato hybrids through protoplast fusions and analysis of their performance in the field.

Authors:  W M Mattheij; K J Puite
Journal:  Theor Appl Genet       Date:  1992-04       Impact factor: 5.699

6.  Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes.

Authors:  Y S Xu; M G Jones; A Karp; E Pehu
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

7.  Expression of reducing sugar accumulation in interspecific somatic hybrids of potato.

Authors:  A L Craig; I Morrison; E Baird; R Waugh; M Coleman; P Davie; W Powell
Journal:  Plant Cell Rep       Date:  1994-04       Impact factor: 4.570

8.  Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion.

Authors:  M Stattmann; E Gerick; G Wenzel
Journal:  Plant Cell Rep       Date:  1994-01       Impact factor: 4.570

9.  A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solatium tuberosum L.).

Authors:  S Waara; L Pijnacker; M A Ferwerda; A Wallin; T Eriksson
Journal:  Theor Appl Genet       Date:  1992-12       Impact factor: 5.699

10.  Somatic fusion for combining virus resistances in Solanum tuberosum L.

Authors:  N Q Thach; U Frei; G Wenzel
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699
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