Literature DB >> 24241421

Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae.

J Fahleson1, J Dixelius, E Sundberg, K Glimelius.   

Abstract

Fourty-one somatic hybrids from two species combinations, Brassica oleracea + B. campestris and B. napus + Eruca sativa, were analysed for chromosome number and nuclear DNA content. The DNA content was measured in a flow cytometer using two internal standards as references and when related to the chromosome number a correlation of 0.91 was found. The chromosome number of the hybrids could be determined with an accuracy of ±10% by using flow cytometry, and the smallest statistically significant difference in DNA content between two individuals was 0.23 pg DNA/cell.

Entities:  

Year:  1988        PMID: 24241421     DOI: 10.1007/BF00272983

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN: 0721-7714            Impact factor:   4.570


  8 in total

1.  Rapid flow cytometric analysis of the cell cycle in intact plant tissues.

Authors:  D W Galbraith; K R Harkins; J M Maddox; N M Ayres; D P Sharma; E Firoozabady
Journal:  Science       Date:  1983-06-03       Impact factor: 47.728

2.  Flow cytometric analysis of polysomaty and in vitro genetic instability in potato.

Authors:  K S Ramulu; P Dijkhuis
Journal:  Plant Cell Rep       Date:  1986-06       Impact factor: 4.570

3.  A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis.

Authors:  L L Vindeløv; I J Christensen; N I Nissen
Journal:  Cytometry       Date:  1983-03

4.  Long-term storage of samples for flow cytometric DNA analysis.

Authors:  L L Vindeløv; I J Christensen; N Keiding; M Spang-Thomsen; N I Nissen
Journal:  Cytometry       Date:  1983-03

5.  Standardization of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards.

Authors:  L L Vindeløv; I J Christensen; N I Nissen
Journal:  Cytometry       Date:  1983-03

6.  Somatic hybrid potato plants after electrofusion of diploid Solanum tuberosum and Solanum phureja.

Authors:  K J Puite; S Roest; L P Pijnacker
Journal:  Plant Cell Rep       Date:  1986-08       Impact factor: 4.570

7.  Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability.

Authors:  R Terada; Y Yamashita; S Nishibayashi; K Shimamoto
Journal:  Theor Appl Genet       Date:  1987-01       Impact factor: 5.699

8.  Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

Authors:  L Menczel; F Nagy; Z R Kiss; P Maliga
Journal:  Theor Appl Genet       Date:  1981-03       Impact factor: 5.699

  8 in total
  14 in total

1.  Rapid detection of aneuploidy in Musa using flow cytometry.

Authors:  N Roux; A Toloza; Z Radecki; F J Zapata-Arias; J Dolezel
Journal:  Plant Cell Rep       Date:  2002-12-10       Impact factor: 4.570

2.  Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja.

Authors:  L P Pijnacker; M A Ferwerda; K J Puite; J G Schaart
Journal:  Plant Cell Rep       Date:  1989-02       Impact factor: 4.570

3.  Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae.

Authors:  E Sundberg; K Glimelius
Journal:  Theor Appl Genet       Date:  1991-11       Impact factor: 5.699

4.  Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii).

Authors:  K Miyoshi; N Asakura
Journal:  Plant Cell Rep       Date:  1996-11       Impact factor: 4.570

5.  Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa.

Authors:  J Fahleson; L Råhlén; K Glimelius
Journal:  Theor Appl Genet       Date:  1988-10       Impact factor: 5.699

6.  Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis.

Authors:  S Waara; H Tegelström; A Wallin; T Eriksson
Journal:  Theor Appl Genet       Date:  1989-01       Impact factor: 5.699

7.  Morphogenic and genetic stability in longterm embryogenic cultures and somatic embryos of Norway spruce (Picea abies {L.} Karst).

Authors:  L H Mo; S von Arnold; U Lagercrantz
Journal:  Plant Cell Rep       Date:  1989-10       Impact factor: 4.570

8.  Brassica naponigra, a somatic hybrid resistant to Phoma lingam.

Authors:  C Sjödin; K Glimelius
Journal:  Theor Appl Genet       Date:  1989-05       Impact factor: 5.699

9.  Production and characterization of fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L.

Authors:  M C Daunay; M H Chaput; D Sihachakr; M Allot; F Vedel; G Ducreux
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

10.  Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid.

Authors:  J Fahleson; I Eriksson; M Landgren; S Stymne; K Glimelius
Journal:  Theor Appl Genet       Date:  1994-02       Impact factor: 5.699

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.