Literature DB >> 24227081

Assessment of murine neuroblastoma (N1E-115) resting membrane potential by confocal microscopy.

M Hernandez1, W S Kisaalita, M A Farmer.   

Abstract

Digital imaging (confocal microscopy) and a slow potentiometric dye (tetramethylrhodamine methyl ester) were used to assess the resting membrane potential (V m) of murine neuroblastoma cells (N1E-115). The averageV m was found to be -64.0±2.0 mV. The difference between this and the previously reported higher values was attributed to the use of glass microelectrode techniques that probably caused mechanical injury to the cell membranes: Digital imaging of N1E-115V m was found to be sensitive, reproducible, fast, and simple.

Entities:  

Year:  1996        PMID： 24227081     DOI： 10.1007/BF00732046

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  8 in total

1.  Maturation of neuroblastoma cells in the presence of dimethylsulfoxide.

Authors:  Y Kimhi; C Palfrey; I Spector; Y Barak; U Z Littauer
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

2.  Enhancement of the electrical excitability of neuroblastoma cells by valinomycin.

Authors:  I Spector; C Palfrey; U Z Littauer
Journal:  Nature       Date:  1975-03-13       Impact factor: 49.962

3.  Induction of temporally dissociated morphological and physiological differentiation of N1E-115 cells.

Authors:  C Cosgrove; P Cobbett
Journal:  Brain Res Bull       Date:  1991-07       Impact factor: 4.077

4.  Anthracycline-induced inhibition of a calcium action potential in differentiated murine neuroblastoma cells.

Authors:  K S Santone; S G Oakes; S R Taylor; G Powis
Journal:  Cancer Res       Date:  1986-06       Impact factor: 12.701

5.  Membrane potential can be determined in individual cells from the nernstian distribution of cationic dyes.

Authors:  B Ehrenberg; V Montana; M D Wei; J P Wuskell; L M Loew
Journal:  Biophys J       Date:  1988-05       Impact factor: 4.033

6.  Enhancement in excitability properties of mouse neuroblastoma cells cultured in the presence of dibutyryl cyclic AMP.

Authors:  A Chalazonitis; L A Greene
Journal:  Brain Res       Date:  1974-06-07       Impact factor: 3.252

7.  Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties.

Authors:  R K Emaus; R Grunwald; J J Lemasters
Journal:  Biochim Biophys Acta       Date:  1986-07-23

8.  A miniature flow cell designed for rapid exchange of media under high-power microscope objectives.

Authors:  H C Berg; S M Block
Journal:  J Gen Microbiol       Date:  1984-11
  8 in total
  3 in total

1.  Effect of medium serum concentration on N1E-115 neuroblastoma membrane potential development.

Authors:  W S Kisaalita; J M Bowen
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-03       Impact factor: 2.416

2.  Biochemical and electrophysiological differentiation profile of a human neuroblastoma (IMR-32) cell line.

Authors:  Raj R Rao; William S Kisaalita
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-09       Impact factor: 2.416

3.  Modeling the electric potential across neuronal membranes: the effect of fixed charges on spinal ganglion neurons and neuroblastoma cells.

Authors:  Thiago M Pinto; Roseli S Wedemann; Célia M Cortez
Journal:  PLoS One       Date:  2014-05-06       Impact factor: 3.240
  3 in total

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