Literature DB >> 24226815

Transgenic Brassica napus plants obtained by cocultivation of protoplasts with Agrobacterium tumefaciens.

J E Thomzik1, R Hain.   

Abstract

Hypocotyl protoplasts of German winter oilseed, rape (Brassica napus) lines of double-low quality were transformed using Agrobacterium tumefaciens harbouring pGV 3850∶1103 neo (dimer) containing chimaeric kanamycin resistance reporter genes. Transformed protoplasts were regenerated to fertile and phenotypically normal plants. Transformation was confirmed by kanamycin resistance, nopaline production, neomycinphosphotransferase II activity, and Southern blot hybridization. Seed progeny from self-pollinated transformants expressed the introduced kanamycin resistance as a Mendelian trait.

Entities:  

Year:  1990        PMID： 24226815     DOI： 10.1007/BF00232290

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  9 in total

1.  Transformation of Brassica napus with Agrobacterium tumefaciens based vectors.

Authors:  J Fry; A Barnason; R B Horsch
Journal:  Plant Cell Rep       Date:  1987-10       Impact factor: 4.570

2.  High efficiency transformation ofBrassica napus usingAgrobacterium vectors.

Authors:  M M Moloney; J M Walker; K K Sharma
Journal:  Plant Cell Rep       Date:  1989-04       Impact factor: 4.570

3.  Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites.

Authors:  S H Hughes; P R Shank; D H Spector; H J Kung; J M Bishop; H E Varmus; P K Vogt; M L Breitman
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

4.  A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors:  B Reiss; R Sprengel; H Will; H Schaller
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

5.  Transformation of Brassica napus and Brassica oleracea Using Agrobacterium tumefaciens and the Expression of the bar and neo Genes in the Transgenic Plants.

Authors:  M De Block; D De Brouwer; P Tenning
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

6.  Organ-specific and developmental regulation of the nopaline synthase promoter in transgenic tobacco plants.

Authors:  G An; M A Costa; A Mitra; S B Ha; L Márton
Journal:  Plant Physiol       Date:  1988-11       Impact factor: 8.340

7.  Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L.

Authors:  J E Thomzik; R Hain
Journal:  Theor Appl Genet       Date:  1988-08       Impact factor: 5.699

8.  Fate of selectable marker DNA integrated into the genome of Nicotiana tabacum.

Authors:  A P Czernilofsky; R Hain; L Herrera-Estrella; H Lörz; E Goyvaerts; B J Baker; J Schell
Journal:  DNA       Date:  1986-04

9.  Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion.

Authors:  M D Sacristán; M Gerdemann-Knörck; O Schieder
Journal:  Theor Appl Genet       Date:  1989-08       Impact factor: 5.699
  9 in total
  3 in total

1.  Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake.

Authors:  A Mukhopadhyay; R Töpfer; A K Pradhan; Y S Sodhi; H H Steinbiß; J Schell; D Pental
Journal:  Plant Cell Rep       Date:  1991-10       Impact factor: 4.570

2.  Genetic transformation of Brassica nigra by agrobacterium based vector and direct plasmid uptake.

Authors:  V Gupta; G Lakshmi Sita; M S Shaila; V Jagannathan
Journal:  Plant Cell Rep       Date:  1993-05       Impact factor: 4.570

3.  Rapid and efficient plant regeneration from hypocotyl protoplasts of Brassica carinata.

Authors:  S B Narasimhulu; P B Kirti; S Prakash; V L Chopra
Journal:  Plant Cell Rep       Date:  1992-04       Impact factor: 4.570
  3 in total

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