The cytological and genetic characterisation of doubled haploid lines derived from triticale×wheat hybrids.

Anther culture, when applied to hexaploid triticale×wheat hybrids, offers the opportunity to re-assort wheat D genome and rye R genome chromosomes into homozygous doubled haploid lines in a single generation. The characterisation of such lines is the first step in their utilisation in wheat improvement. Two lines, M24 and M25 from the cross of 'Beagle'×'Kedong 58', and one line, M27, from the cross 'Beagle'×'Jinghua No. 1' have been characterised using different methods including conventional cytology and chromosome banding, and by using marker systems for storage protein composition (glutenins and gliadins), isozymes (α-amylase, aminopeptidase, glutamate oxaloacetic transaminase (GOT)) and RFLP markers. The results from all approaches were consistent in proving that M24 is a whole chromosome 6R/6D substitution line, while M25 and M27 are whole chromosome 1R/1D substitution lines. The relative advantages and disadvantages of each method of identification are also discussed.