| Literature DB >> 24216106 |
Xueming Cao1, Aihua Chen, Pingzhen Yang, Xudong Song, Yingfeng Liu, Zhiliang Li, Xianbao Wang, Lizi Wang, Yunpeng Li.
Abstract
Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Alpha-lipoic acid (LA) plays an important role in the etiology of cardiovascular disease. Autophagy is widely implicated in myocardial I/R injury. We assessed the degree of autophagy by pretreatment with LA exposed to H/R in H9c2 cell based on the expression levels of Beclin-1, LC3II/LC3I, and green fluorescent protein-labeled LC3 fusion proteins. Autophagic vacuoles were confirmed in H9c2 cells exposed to H/R using transmission electron microscopy. Our findings indicated that pretreatment with LA inhibited the degree of autophagy in parallel to the enhanced cell survival and decreased total cell death in H9c2 cells exposed to H/R. We conclude that LA protects cardiomyocytes against H/R injury by inhibiting autophagy.Entities:
Keywords: Alpha-lipoic acid; Autophagy; Cardiomyocytes; Hypoxia/reoxygenation
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Year: 2013 PMID： 24216106 DOI： 10.1016/j.bbrc.2013.10.166
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575