Literature DB >> 24214306

An electrospray ionization mass spectrometry study of copper adducts of protonated ubiquitin.

C Q Jiao1, B S Freiser, S R Carr, C J Cassady.   

Abstract

A study of the addition of Cu(II) to a ubiquitin electrospray solution shows that the copper ion in the ubiquitin remains doubly charged and displaces two protons on the protonated protein molecule. This observation indicates a chelating bond between the protein and the Cu(II) species. The addition of Cu(I) also was studied and significant intensity was observed for adducts with up to four Cu(I) species attached, with each Cu(I) bonded to one basic site on the protein.

Entities:  

Year:  1995        PMID： 24214306     DOI： 10.1016/1044-0305(95)00198-M

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  9 in total

1.  Charge determination of product ions formed from collision-induced dissociation of multiply protonated molecules via ion/molecule reactions.

Authors:  S A McLuckey; G L Glish; G J Van Berkel
Journal:  Anal Chem       Date:  1991-09-15       Impact factor: 6.986

2.  Electrospray ionization mass spectrometry: Deconvolution by an Entropy-Based algorithm.

Authors:  B B Reinhold; V N Reinhold
Journal:  J Am Soc Mass Spectrom       Date:  1992-03       Impact factor: 3.109

3.  Studies on heme binding in myoglobin, hemoglobin, and cytochrome c by ion spray mass spectrometry.

Authors:  Y T Li; Y L Hsieh; J D Henion; B Ganem
Journal:  J Am Soc Mass Spectrom       Date:  1993-08       Impact factor: 3.109

4.  Mass and charge assignment for electrospray ions by cation adduction.

Authors:  M W Senko; S C Beu; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1993-10       Impact factor: 3.109

5.  Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry.

Authors:  V Katta; B T Chait
Journal:  Rapid Commun Mass Spectrom       Date:  1991-04       Impact factor: 2.419

6.  Differences in the conformational state of a zinc-finger DNA-binding protein domain occupied by zinc and copper revealed by electrospray ionization mass spectrometry.

Authors:  T W Hutchens; M H Allen
Journal:  Rapid Commun Mass Spectrom       Date:  1992-07       Impact factor: 2.419

7.  Capillary electrophoresis/mass spectrometry determination of inorganic ions using an ion spray-sheath flow interface.

Authors:  T G Huggins; J D Henion
Journal:  Electrophoresis       Date:  1993 May-Jun       Impact factor: 3.535

8.  Deprotonation reactions of multiply protonated ubiquitin ions.

Authors:  C J Cassady; J Wronka; G H Kruppa; F H Laukien
Journal:  Rapid Commun Mass Spectrom       Date:  1994-05       Impact factor: 2.419

9.  Occupancy of a C2-C2 type 'zinc-finger' protein domain by copper. Direct observation by electrospray ionization mass spectrometry.

Authors:  T W Hutchens; M H Allen; C M Li; T T Yip
Journal:  FEBS Lett       Date:  1992-09-07       Impact factor: 4.124
  9 in total
  1 in total

1.  A new copper containing MALDI matrix that yields high abundances of [peptide + Cu]+ ions.

Authors:  Zhaoxiang Wu; Francisco A Fernandez-Lima; Lisa M Perez; David H Russell
Journal:  J Am Soc Mass Spectrom       Date:  2009-02-25       Impact factor: 3.109
  1 in total

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