Literature DB >> 1666528

Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry.

V Katta1, B T Chait.   

Abstract

Hydrogen-exchange electrospray-ionization mass spectrometry is demonstrated to be an effective new method for probing conformational changes of proteins in solutions. The method is based on the mass spectrometric measurement of the extent of hydrogen/deuterium exchange that occurs in different protein conformers over defined periods of time. Results are presented in which hydrogen-exchange electrospray-ionization mass spectrometry is used to probe conformational changes in bovine ubiquitin induced by the addition of methanol to aqueous acidic solutions of the protein.

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Year:  1991        PMID： 1666528     DOI： 10.1002/rcm.1290050415

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  109 in total

1.  Quench-flow experiments combined with mass spectrometry show apomyoglobin folds through and obligatory intermediate.

Authors:  V Tsui; C Garcia; S Cavagnero; G Siuzdak; H J Dyson; P E Wright
Journal:  Protein Sci       Date:  1999-01       Impact factor: 6.725

2.  H/D exchange of gas phase bradykinin ions in a linear quadrupole ion trap.

Authors:  Dunmin Mao; D J Douglas
Journal:  J Am Soc Mass Spectrom       Date:  2003-02       Impact factor: 3.109

3.  Inter-molecular migration during collisional activation monitored by hydrogen/deuterium exchange FT-ICR tandem mass spectrometry.

Authors:  Charlotte Hagman; Per Håkansson; Jos Buijs; Kristina Håkansson
Journal:  J Am Soc Mass Spectrom       Date:  2004-05       Impact factor: 3.109

4.  Elucidating the higher-order structure of biopolymers by structural probing and mass spectrometry: MS3D.

Authors:  Daniele Fabris; Eizadora T Yu
Journal:  J Mass Spectrom       Date:  2010-08       Impact factor: 1.982

5.  Interactions of apurinic/apyrimidinic endonuclease with a redox inhibitor: evidence for an alternate conformation of the enzyme.

Authors:  Dian Su; Sarah Delaplane; Meihua Luo; Don L Rempel; Bich Vu; Mark R Kelley; Michael L Gross; Millie M Georgiadis
Journal:  Biochemistry       Date:  2010-12-08       Impact factor: 3.162

6.  Resolving isotopic fine structure to detect and quantify natural abundance- and hydrogen/deuterium exchange-derived isotopomers.

Authors:  Qian Liu; Michael L Easterling; Jeffrey N Agar
Journal:  Anal Chem       Date:  2013-12-20       Impact factor: 6.986

7.  An electrospray ionization mass spectrometry study of copper adducts of protonated ubiquitin.

Authors:  C Q Jiao; B S Freiser; S R Carr; C J Cassady
Journal:  J Am Soc Mass Spectrom       Date:  1995-06       Impact factor: 3.109

8.  Characterization of disulfide linkages and disulfide bond scrambling in recombinant human macrophage colony stimulating factor by fast-atom bombardment mass spectrometry of enzymatic digests.

Authors:  M O Glocker; B Arbogast; M L Deinzer
Journal:  J Am Soc Mass Spectrom       Date:  1995-08       Impact factor: 3.109

9.  Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry.

Authors:  Alem W Kahsai; Sudarshan Rajagopal; Jinpeng Sun; Kunhong Xiao
Journal:  Nat Protoc       Date:  2014-05-08       Impact factor: 13.491

10.  Gas-phase folding and unfolding of cytochrome c cations.

Authors:  T D Wood; R A Chorush; F M Wampler; D P Little; P B O'Connor; F W McLafferty
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205
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