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Literature DB >> 24213286

Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants.

N Assad-García¹, N Ochoa-Alejo, E García-Hernández, L Herrera-Estrella, J Simpson.

Abstract

A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.

Entities: Species

Year: 1992 PMID： 24213286 DOI： 10.1007/BF00233092

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

8 in total

1. Regulated genes in transgenic plants.

Authors: P N Benfey; N H Chua
Journal: Science Date: 1989-04-14 Impact factor: 47.728

2. Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.

Authors: G Van den Broeck; M P Timko; A P Kausch; A R Cashmore; M Van Montagu; L Herrera-Estrella
Journal: Nature Date: 1985 Jan 31-Feb 6 Impact factor: 49.962

3. A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors: B Reiss; R Sprengel; H Will; H Schaller
Journal: Gene Date: 1984-10 Impact factor: 3.688

4. Differential Accumulation of a Transcript Driven by the CaMV 35S Promoter in Transgenic Tobacco.

Authors: J D Williamson; M E Hirsch-Wyncott; B A Larkins; S B Gelvin
Journal: Plant Physiol Date: 1989-08 Impact factor: 8.340

5. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter.

Authors: J T Odell; F Nagy; N H Chua
Journal: Nature Date: 1985 Feb 28-Mar 6 Impact factor: 49.962

6. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.

Authors: P P Abel; R S Nelson; B De; N Hoffmann; S G Rogers; R T Fraley; R N Beachy
Journal: Science Date: 1986-05-09 Impact factor: 47.728

7. The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns.

Authors: P N Benfey; L Ren; N H Chua
Journal: EMBO J Date: 1989-08 Impact factor: 11.598

8. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors: R A Jefferson; T A Kavanagh; M W Bevan
Journal: EMBO J Date: 1987-12-20 Impact factor: 11.598

8 in total

5 in total

5. Heterotopic expression of MPF2 is the key to the evolution of the Chinese lantern of Physalis, a morphological novelty in Solanaceae.

Authors: Chaoying He; Heinz Saedler
Journal: Proc Natl Acad Sci U S A Date: 2005-04-11 Impact factor: 11.205