Literature DB >> 16666967

Differential Accumulation of a Transcript Driven by the CaMV 35S Promoter in Transgenic Tobacco.

J D Williamson1, M E Hirsch-Wyncott, B A Larkins, S B Gelvin.   

Abstract

A transcriptional fusion composed of the CaMV 35S promoter, a 19 kD zein cDNA, and the 3' flanking regions from the 0' to 1' T(R) T-DNA genes was introduced into tobacco (Nicotiana tabacum) by Agrobacterium-mediated transformation. The accumulation of RNA generated from this transcriptional fusion varied both temporally and spatially in all tissues examined in greenhouse-grown tobacco plants, suggesting that the CaMV 35S promoter is not constitutive. Younger, actively dividing leaf, stem, root, and flower tissues contained higher steady state levels of zein RNA than did older, more quiescent tissues. Zein RNA levels greatly decreased during seed development and were undetectable in the mature seed. In addition, the two RNA termination or processing signals present in our construct were differentially utilized during seed development.

Entities:  

Year:  1989        PMID： 16666967      PMCID： PMC1061927          DOI： 10.1104/pp.90.4.1570

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  14 in total

1.  The synthesis of a 19 kilodalton zein protein in transgenic petunia plants.

Authors:  J D Williamson; G Galili; B A Larkins; S B Gelvin
Journal:  Plant Physiol       Date:  1988-12       Impact factor: 8.340

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Nucleotide sequence analysis of zein mRNAs from maize endosperm.

Authors:  M D Marks; J S Lindell; B A Larkins
Journal:  J Biol Chem       Date:  1985-12-25       Impact factor: 5.157

4.  Organ-specific and developmental regulation of the nopaline synthase promoter in transgenic tobacco plants.

Authors:  G An; M A Costa; A Mitra; S B Ha; L Márton
Journal:  Plant Physiol       Date:  1988-11       Impact factor: 8.340

5.  Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter.

Authors:  J T Odell; F Nagy; N H Chua
Journal:  Nature       Date:  1985 Feb 28-Mar 6       Impact factor: 49.962

6.  Binary Agrobacterium vectors for plant transformation.

Authors:  M Bevan
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

7.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

8.  Synthetic oligonucleotide tails inhibit in vitro and in vivo translation of SP6 transcripts of maize zein cDNA clones.

Authors:  G Galili; E E Kawata; R E Cuellar; L D Smith; B A Larkins
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

9.  A simple and general method for transferring genes into plants.

Authors: 
Journal:  Science       Date:  1985-03-08       Impact factor: 47.728

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
View more
  28 in total

1.  Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a.

Authors:  Zeng-Fu Xu; Whei-Lan Teng; Mee-Len Chye
Journal:  Planta       Date:  2003-10-23       Impact factor: 4.116

2.  Expression of a chimeric CaMV 35S Bacillus thuringiensis insecticidal protein gene in transgenic tobacco.

Authors:  N B Carozzi; G W Warren; N Desai; S M Jayne; R Lotstein; D A Rice; S Evola; M G Koziel
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

3.  Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP.

Authors:  Ganesan Sunilkumar; LeAnne Mohr; Emily Lopata-Finch; Chandrakanth Emani; Keerti S Rathore
Journal:  Plant Mol Biol       Date:  2002-10       Impact factor: 4.076

4.  Translation controls the expression level of a chimaeric reporter gene.

Authors:  L A Hensgens; M W Fornerod; S Rueb; A A Winkler; S van der Veen; R A Schilperoort
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

Review 5.  From miracle fruit to transgenic tomato: mass production of the taste-modifying protein miraculin in transgenic plants.

Authors:  Kyoko Hiwasa-Tanase; Tadayoshi Hirai; Kazuhisa Kato; Narendra Duhita; Hiroshi Ezura
Journal:  Plant Cell Rep       Date:  2011-12-08       Impact factor: 4.570

6.  The in vivo pattern of firefly luciferase expression in transgenic plants.

Authors:  M Schneider; D W Ow; S H Howell
Journal:  Plant Mol Biol       Date:  1990-06       Impact factor: 4.076

7.  The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants.

Authors:  S L Hobbs; P Kpodar; C M DeLong
Journal:  Plant Mol Biol       Date:  1990-12       Impact factor: 4.076

8.  Stability of the MON 810 transgene in maize.

Authors:  Jose Luis La Paz; Maria Pla; Nina Papazova; Pere Puigdomènech; Carlos M Vicient
Journal:  Plant Mol Biol       Date:  2010-10-09       Impact factor: 4.076

9.  Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA.

Authors:  O. Nilsson; T. Moritz; N. Imbault; G. Sandberg; O. Olsson
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

10.  Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants.

Authors:  N Assad-García; N Ochoa-Alejo; E García-Hernández; L Herrera-Estrella; J Simpson
Journal:  Plant Cell Rep       Date:  1992-10       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.